Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5392930..5393555 Replicon chromosome
Accession NZ_CP098335
Organism Klebsiella pneumoniae strain 53023_J2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NB665_RS26525 Protein ID WP_002882817.1
Coordinates 5392930..5393313 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NB665_RS26530 Protein ID WP_004150355.1
Coordinates 5393313..5393555 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB665_RS26510 (NB665_26510) 5390296..5391198 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NB665_RS26515 (NB665_26515) 5391195..5391830 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NB665_RS26520 (NB665_26520) 5391827..5392756 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NB665_RS26525 (NB665_26525) 5392930..5393313 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NB665_RS26530 (NB665_26530) 5393313..5393555 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NB665_RS26535 (NB665_26535) 5393760..5394677 + 918 WP_002882812.1 alpha/beta hydrolase -
NB665_RS26540 (NB665_26540) 5394691..5395632 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NB665_RS26545 (NB665_26545) 5395677..5396114 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NB665_RS26550 (NB665_26550) 5396111..5396971 - 861 WP_002882807.1 virulence factor BrkB family protein -
NB665_RS26555 (NB665_26555) 5396965..5397564 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T247447 WP_002882817.1 NZ_CP098335:c5393313-5392930 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT247447 WP_004150355.1 NZ_CP098335:c5393555-5393313 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References