Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5007103..5007719 Replicon chromosome
Accession NZ_CP098330
Organism Citrobacter freundii strain 59174

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag NB664_RS24565 Protein ID WP_003028682.1
Coordinates 5007103..5007477 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag NB664_RS24570 Protein ID WP_043018956.1
Coordinates 5007477..5007719 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB664_RS24550 (5004606) 5004606..5005508 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
NB664_RS24555 (5005505) 5005505..5006140 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
NB664_RS24560 (5006137) 5006137..5007066 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
NB664_RS24565 (5007103) 5007103..5007477 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
NB664_RS24570 (5007477) 5007477..5007719 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
NB664_RS24575 (5007925) 5007925..5008833 + 909 WP_044701816.1 alpha/beta hydrolase -
NB664_RS24580 (5008984) 5008984..5009925 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
NB664_RS24585 (5009970) 5009970..5010407 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
NB664_RS24590 (5010404) 5010404..5011276 - 873 WP_003028669.1 virulence factor BrkB family protein -
NB664_RS24595 (5011270) 5011270..5011869 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T247431 WP_003028682.1 NZ_CP098330:c5007477-5007103 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT247431 WP_043018956.1 NZ_CP098330:c5007719-5007477 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References