Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4118123..4118802 Replicon chromosome
Accession NZ_CP098330
Organism Citrobacter freundii strain 59174

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag NB664_RS20335 Protein ID WP_003031349.1
Coordinates 4118461..4118802 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag NB664_RS20330 Protein ID WP_003031347.1
Coordinates 4118123..4118440 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NB664_RS20305 (4114216) 4114216..4116213 - 1998 WP_016245729.1 choline BCCT transporter BetT -
NB664_RS20310 (4116761) 4116761..4116908 + 148 Protein_3990 hypothetical protein -
NB664_RS20315 (4116922) 4116922..4117383 + 462 WP_003031344.1 antirestriction protein -
NB664_RS20320 (4117399) 4117399..4117875 + 477 WP_003031345.1 RadC family protein -
NB664_RS20325 (4117884) 4117884..4118105 + 222 WP_003031346.1 DUF987 domain-containing protein -
NB664_RS20330 (4118123) 4118123..4118440 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
NB664_RS20335 (4118461) 4118461..4118802 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
NB664_RS20340 (4119392) 4119392..4119994 - 603 WP_224770543.1 tail fiber assembly protein -
NB664_RS20345 (4119996) 4119996..4121243 - 1248 WP_250791465.1 hypothetical protein -
NB664_RS20350 (4121447) 4121447..4122127 - 681 WP_121927014.1 DUF2612 domain-containing protein -
NB664_RS20355 (4122124) 4122124..4123323 - 1200 WP_121927013.1 baseplate J/gp47 family protein -
NB664_RS20360 (4123324) 4123324..4123677 - 354 WP_016239889.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4116922..4161317 44395


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T247428 WP_003031349.1 NZ_CP098330:4118461-4118802 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT247428 WP_003031347.1 NZ_CP098330:4118123-4118440 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References