Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1265061..1265681 Replicon chromosome
Accession NC_014121
Organism Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag ECL_RS06000 Protein ID WP_013095889.1
Coordinates 1265061..1265279 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag ECL_RS06005 Protein ID WP_008499288.1
Coordinates 1265307..1265681 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECL_RS05970 1261073..1261333 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
ECL_RS05975 1261336..1261476 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
ECL_RS05980 1261473..1262183 - 711 WP_013095885.1 GNAT family N-acetyltransferase -
ECL_RS05985 1262285..1263745 + 1461 WP_013095886.1 PLP-dependent aminotransferase family protein -
ECL_RS05990 1263717..1264184 - 468 WP_013095887.1 membrane protein -
ECL_RS05995 1264300..1264851 - 552 WP_013095888.1 maltose O-acetyltransferase -
ECL_RS06000 1265061..1265279 - 219 WP_013095889.1 hemolysin expression modulator Hha Toxin
ECL_RS06005 1265307..1265681 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
ECL_RS06010 1266194..1269340 - 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit -
ECL_RS06015 1269363..1270556 - 1194 WP_013095891.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T24741 WP_013095889.1 NC_014121:c1265279-1265061 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T24741 NC_014121:c1265279-1265061 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACACTCGAACGCGT
GATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACCGTATTTTATTCAGCCGCTGACCATCGCCTTGCCGAGC
TGACCATGAATAAACTGTATGACAAAATCCCTCCTTCCGTGTGGAAATTTGTCCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT24741 WP_008499288.1 NC_014121:c1265681-1265307 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT24741 NC_014121:c1265681-1265307 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTGAACGATCCAACATCGGCCATCAATTTGCAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References