Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 252380..253016 Replicon chromosome
Accession NZ_CP098323
Organism Cytobacillus firmus strain PheN7

Toxin (Protein)


Gene name mazF Uniprot ID W7KPR3
Locus tag NAF01_RS01360 Protein ID WP_009336311.1
Coordinates 252666..253016 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID W7L1R3
Locus tag NAF01_RS01355 Protein ID WP_035331931.1
Coordinates 252380..252661 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAF01_RS01335 (NAF01_01335) 247951..248676 - 726 WP_250801583.1 rhomboid family intramembrane serine protease -
NAF01_RS01340 (NAF01_01340) 248832..249185 + 354 WP_048008767.1 holo-ACP synthase -
NAF01_RS01345 (NAF01_01345) 249330..250343 + 1014 WP_197212719.1 outer membrane lipoprotein carrier protein LolA -
NAF01_RS01350 (NAF01_01350) 250881..252035 + 1155 WP_226620280.1 alanine racemase -
NAF01_RS01355 (NAF01_01355) 252380..252661 + 282 WP_035331931.1 YlcI/YnfO family protein Antitoxin
NAF01_RS01360 (NAF01_01360) 252666..253016 + 351 WP_009336311.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NAF01_RS01365 (NAF01_01365) 253391..254224 + 834 WP_163144748.1 RsbT co-antagonist protein RsbRA -
NAF01_RS01370 (NAF01_01370) 254221..254583 + 363 WP_009336309.1 STAS domain-containing protein -
NAF01_RS01375 (NAF01_01375) 254588..254989 + 402 WP_048008772.1 anti-sigma regulatory factor -
NAF01_RS01380 (NAF01_01380) 255001..256011 + 1011 WP_048008773.1 PP2C family protein-serine/threonine phosphatase -
NAF01_RS01385 (NAF01_01385) 256072..256404 + 333 WP_035331808.1 anti-sigma factor antagonist -
NAF01_RS01390 (NAF01_01390) 256401..256874 + 474 WP_009336304.1 anti-sigma B factor RsbW -
NAF01_RS01395 (NAF01_01395) 256852..257646 + 795 WP_048008774.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13006.03 Da        Isoelectric Point: 4.8998

>T247404 WP_009336311.1 NZ_CP098323:252666-253016 [Cytobacillus firmus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEAVQISLGLIEF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10831.33 Da        Isoelectric Point: 7.2028

>AT247404 WP_035331931.1 NZ_CP098323:252380-252661 [Cytobacillus firmus]
VSESSTTTEIMVKLPQHLLTELDGFAKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A169FD46

Antitoxin

Source ID Structure
AlphaFold DB W7L1R3

References