Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 282883..283479 Replicon chromosome
Accession NC_014121
Organism Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)


Gene name PumA Uniprot ID A0A0M7H115
Locus tag ECL_RS01340 Protein ID WP_029882644.1
Coordinates 283177..283479 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A0F1AUP1
Locus tag ECL_RS01335 Protein ID WP_013095028.1
Coordinates 282883..283170 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECL_RS01330 281255..282886 + 1632 WP_013095027.1 Na/Pi cotransporter family protein -
ECL_RS01335 282883..283170 - 288 WP_013095028.1 putative addiction module antidote protein Antitoxin
ECL_RS01340 283177..283479 - 303 WP_029882644.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECL_RS01345 283677..284546 + 870 WP_013095030.1 23S rRNA pseudouridine(2604) synthase RluF -
ECL_RS01350 284614..285558 - 945 WP_013095031.1 ketopantoate/pantoate/pantothenate transporter PanS -
ECL_RS01355 285651..287000 - 1350 WP_013095032.1 lysine-sensitive aspartokinase 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-93)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11457.30 Da        Isoelectric Point: 9.7662

>T24737 WP_029882644.1 NC_014121:c283479-283177 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MKEIVQTESFRRWEQNLKDRRARTIIASRLFRLANGLAGDIKPVGEGICELRIHFGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Download         Length: 303 bp

>T24737 NC_014121:c283479-283177 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAAGATCGGCGAGCAAGGACCATTATCGC
GTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACATAAAACCCGTGGGGGAAGGTATATGCGAGCTGAGGATCC
ACTTTGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10070.62 Da        Isoelectric Point: 5.7807

>AT24737 WP_013095028.1 NC_014121:c283170-282883 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MHKLTPYDPAIALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPAGD

Download         Length: 288 bp

>AT24737 NC_014121:c283170-282883 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGCATAAATTAACACCCTACGATCCAGCCATCGCGTTGGTGGATGATGAGGAAATAGCCGTGTTTATGGCTGATGCATT
AGAAACCGGTGACTCAGCGTATATTGCTAAAGCTTTGGGCGTCATCGCGCGAGCGAAAGGGATGTCGACCATTTCACAGC
AAACTGGCCTGTCACGGGAACAACTGTACCGATCATTTAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTCACAATCAAACCCGCCGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M7H115

Antitoxin

Source ID Structure
AlphaFold DB A0A0F1AUP1

References