Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 74701..76324 Replicon plasmid unnamed2
Accession NZ_CP098236
Organism Escherichia coli strain ZYB39

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KFZ69_RS25440 Protein ID WP_000088046.1
Coordinates 74701..76026 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KFZ69_RS25445 Protein ID WP_024136327.1
Coordinates 76031..76324 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFZ69_RS25410 (KFZ69_25360) 70966..71346 + 381 WP_001426317.1 hypothetical protein -
KFZ69_RS25415 (KFZ69_25365) 71404..72069 + 666 WP_000490638.1 hypothetical protein -
KFZ69_RS25425 (KFZ69_25375) 73094..73909 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
KFZ69_RS25430 (KFZ69_25380) 73922..74332 - 411 WP_000985911.1 hypothetical protein -
KFZ69_RS25435 (KFZ69_25385) 74434..74640 - 207 WP_000134171.1 hypothetical protein -
KFZ69_RS25440 (KFZ69_25390) 74701..76026 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KFZ69_RS25445 (KFZ69_25395) 76031..76324 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KFZ69_RS25450 (KFZ69_25400) 76928..77320 - 393 WP_000341066.1 hypothetical protein -
KFZ69_RS25455 (KFZ69_25405) 77693..78034 + 342 WP_001151575.1 hypothetical protein -
KFZ69_RS25460 (KFZ69_25410) 78126..78317 + 192 WP_000398480.1 hypothetical protein -
KFZ69_RS25465 (KFZ69_25415) 78367..78648 + 282 WP_000077926.1 hypothetical protein -
KFZ69_RS25470 78989..79120 - 132 WP_000374059.1 hypothetical protein -
KFZ69_RS25475 (KFZ69_25420) 79211..80452 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / mph(A) / aac(3)-IId / sul1 / qacE / qnrB6 / dfrA27 / ARR-3 / aac(6')-Ib-cr / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B - 1..230802 230802
- flank IS/Tn - - 72535..72810 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T247342 WP_000088046.1 NZ_CP098236:c76026-74701 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT247342 WP_024136327.1 NZ_CP098236:c76324-76031 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References