Detailed information of TA system TA024734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 401432..401994
• Replicon: plasmid pBC201
• Accession: NC_014120
• Organism: Burkholderia sp. CCGE1002

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: D5WNM9
• Locus tag: BC1002_RS35075
• Protein ID: WP_013094683.1
• Coordinates: 401432..401752 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: D5WNN0
• Locus tag: BC1002_RS35080
• Protein ID: WP_013094684.1
• Coordinates: 401746..401994 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC1002_RS35030	396578..398290	-	1713	WP_013094675.1	AAA family ATPase	-
BC1002_RS35035	398443..398652	-	210	WP_013094676.1	hypothetical protein	-
BC1002_RS35040	398662..399081	-	420	WP_013094677.1	DUF2513 domain-containing protein	-
BC1002_RS35050	399371..399877	-	507	WP_013094679.1	hypothetical protein	-
BC1002_RS35055	399856..400332	-	477	WP_013094680.1	hypothetical protein	-
BC1002_RS35060	400356..400727	-	372	WP_013094681.1	hypothetical protein	-
BC1002_RS35070	401069..401413	-	345	WP_013094682.1	hypothetical protein	-
BC1002_RS35075	401432..401752	-	321	WP_013094683.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BC1002_RS35080	401746..401994	-	249	WP_013094684.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BC1002_RS35085	402104..402775	-	672	WP_013094685.1	site-specific integrase	-
BC1002_RS35090	403080..403547	+	468	WP_013094686.1	hypothetical protein	-
BC1002_RS38600	403606..404073	-	468	WP_184144026.1	hypothetical protein	-
BC1002_RS38250	404383..404706	+	324	WP_041736314.1	hypothetical protein	-
BC1002_RS35105	405585..406202	-	618	WP_013094688.1	class I SAM-dependent methyltransferase	-
BC1002_RS37405	406356..406505	-	150	Protein_373	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..489136	489136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-104)

Antitoxin

• SpoVT-AbrB domain (9-45)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11914.16 Da
• Isoelectric Point: 10.5567

>T24734 WP_013094683.1 NC_014120:c401752-401432 [Paraburkholderia atlantica]
MVMRGEVWLVALDPTLGSEIQKTRPCVVVSPPEMHDHLRTVVVAPMTSKGRPAPFRIPVTFKRKQGLILLDQIRAVDKVR
LVKKEGTVADKTLLDTLRTLQEVFAE

Nucleotide

• Length: 321 bp

>T24734 NC_014120:c401752-401432 [Paraburkholderia atlantica]
ATGGTGATGCGCGGGGAAGTCTGGTTGGTCGCGCTCGATCCGACTCTTGGTAGCGAGATTCAAAAGACGCGTCCCTGTGT
CGTCGTTTCGCCCCCCGAAATGCACGACCACTTGCGCACAGTCGTCGTCGCGCCGATGACTTCCAAGGGGCGTCCTGCGC
CGTTTCGGATTCCCGTGACGTTCAAGCGCAAACAGGGCTTGATCCTTCTGGATCAGATTCGCGCAGTAGACAAGGTGCGC
CTGGTCAAGAAAGAGGGCACAGTGGCCGATAAGACGTTGTTGGACACCCTGCGAACCTTGCAGGAAGTCTTTGCAGAATA
G

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8991.20 Da
• Isoelectric Point: 4.4929

>AT24734 WP_013094684.1 NC_014120:c401994-401746 [Paraburkholderia atlantica]
MKTTIRRMGNSQGVLIPKPVLAQLGLESEVEMEVENDAIVLRRPKHKPREGWAEASKAVAESGDDALVMGEFSNADDAEL
AW

Nucleotide

• Length: 249 bp

>AT24734 NC_014120:c401994-401746 [Paraburkholderia atlantica]
ATGAAGACGACGATTCGCAGAATGGGGAACTCGCAGGGCGTGCTAATTCCCAAGCCGGTCCTTGCGCAACTCGGACTGGA
GAGTGAAGTGGAGATGGAAGTCGAGAACGACGCGATTGTTCTGCGCCGTCCGAAGCACAAACCCCGTGAGGGTTGGGCGG
AAGCGAGCAAGGCTGTGGCCGAGAGTGGCGACGATGCATTGGTCATGGGCGAGTTTTCGAATGCCGATGACGCGGAGCTT
GCATGGTGA

Structures

Toxin

• AlphaFold DB: D5WNM9

Antitoxin

• AlphaFold DB: D5WNN0