Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1029160..1029814 Replicon chromosome
Accession NZ_CP098234
Organism Escherichia coli strain ZYB39

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KFZ69_RS05025 Protein ID WP_000244772.1
Coordinates 1029407..1029814 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFZ69_RS05020 Protein ID WP_000354046.1
Coordinates 1029160..1029426 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFZ69_RS04995 (1024329) 1024329..1025072 + 744 WP_000951961.1 SDR family oxidoreductase -
KFZ69_RS05000 (1025129) 1025129..1026562 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
KFZ69_RS05005 (1026607) 1026607..1026918 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KFZ69_RS05010 (1027082) 1027082..1027741 + 660 WP_000250274.1 hemolysin III family protein -
KFZ69_RS05015 (1027937) 1027937..1028917 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
KFZ69_RS05020 (1029160) 1029160..1029426 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFZ69_RS05025 (1029407) 1029407..1029814 + 408 WP_000244772.1 protein YgfX Toxin
KFZ69_RS05030 (1029854) 1029854..1030375 - 522 WP_001055874.1 flavodoxin FldB -
KFZ69_RS05035 (1030487) 1030487..1031383 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFZ69_RS05040 (1031408) 1031408..1032118 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFZ69_RS05045 (1032124) 1032124..1033857 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T247322 WP_000244772.1 NZ_CP098234:1029407-1029814 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT247322 WP_000354046.1 NZ_CP098234:1029160-1029426 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References