Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 31805..32330 Replicon plasmid unnamed2
Accession NZ_CP098233
Organism Escherichia coli strain XJ34

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KFZ60_RS24630 Protein ID WP_001159871.1
Coordinates 32025..32330 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag KFZ60_RS24625 Protein ID WP_000813639.1
Coordinates 31805..32023 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFZ60_RS24610 (27083) 27083..27187 - 105 WP_001443026.1 IS3 family transposase -
KFZ60_RS24615 (27215) 27215..28051 - 837 WP_000222506.1 helix-turn-helix domain-containing protein -
KFZ60_RS24620 (28734) 28734..29699 - 966 Protein_22 IS3 family transposase -
KFZ60_RS24625 (31805) 31805..32023 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KFZ60_RS24630 (32025) 32025..32330 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KFZ60_RS24635 (32331) 32331..33138 + 808 Protein_25 site-specific integrase -
KFZ60_RS24640 (33837) 33837..34592 + 756 WP_000852146.1 replication initiation protein RepE -
KFZ60_RS24645 (35180) 35180..36346 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -
KFZ60_RS24650 (36346) 36346..37317 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..72766 72766


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T247313 WP_001159871.1 NZ_CP098233:32025-32330 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT247313 WP_000813639.1 NZ_CP098233:31805-32023 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References