Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3592397..3593234 Replicon chromosome
Accession NZ_CP098231
Organism Escherichia coli strain XJ34

Toxin (Protein)


Gene name itaT Uniprot ID A0A080JA52
Locus tag KFZ60_RS17375 Protein ID WP_032170811.1
Coordinates 3592692..3593234 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A0A080JFU1
Locus tag KFZ60_RS17370 Protein ID WP_001577859.1
Coordinates 3592397..3592708 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFZ60_RS17345 (3587417) 3587417..3588364 + 948 WP_001239436.1 cytochrome o ubiquinol oxidase subunit II -
KFZ60_RS17350 (3588386) 3588386..3590377 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
KFZ60_RS17355 (3590367) 3590367..3590981 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
KFZ60_RS17360 (3590981) 3590981..3591310 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
KFZ60_RS17365 (3591322) 3591322..3592212 + 891 WP_000971336.1 heme o synthase -
KFZ60_RS17370 (3592397) 3592397..3592708 + 312 WP_001577859.1 DUF1778 domain-containing protein Antitoxin
KFZ60_RS17375 (3592692) 3592692..3593234 + 543 WP_032170811.1 GNAT family N-acetyltransferase Toxin
KFZ60_RS17380 (3593290) 3593290..3594225 - 936 WP_045176456.1 tetratricopeptide repeat protein -
KFZ60_RS17385 (3594632) 3594632..3595996 + 1365 WP_001000956.1 MFS transporter -
KFZ60_RS17390 (3596124) 3596124..3596615 - 492 WP_001138904.1 nucleotide binding protein YajQ -
KFZ60_RS17395 (3596783) 3596783..3597694 + 912 WP_088375888.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espY3 / espY3 3592397..3605815 13418


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19835.05 Da        Isoelectric Point: 8.3395

>T247305 WP_032170811.1 NZ_CP098231:3592692-3593234 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRTSLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11400.08 Da        Isoelectric Point: 10.5548

>AT247305 WP_001577859.1 NZ_CP098231:3592397-3592708 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNRLREAAQAVGLDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A080JA52

Antitoxin

Source ID Structure
AlphaFold DB A0A080JFU1

References