Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 87330..87856 Replicon plasmid pECL_A
Accession NC_014107
Organism Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ECL_RS26310 Protein ID WP_000323025.1
Coordinates 87569..87856 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag ECL_RS26305 Protein ID WP_004196370.1
Coordinates 87330..87569 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECL_RS26280 82598..83974 + 1377 WP_004196363.1 chromate efflux transporter -
ECL_RS26285 84005..84694 - 690 WP_013087175.1 hypothetical protein -
ECL_RS26290 84708..85445 - 738 WP_008460272.1 HupE/UreJ family protein -
ECL_RS26295 85489..85854 - 366 WP_009651956.1 hypothetical protein -
ECL_RS26305 87330..87569 + 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECL_RS26310 87569..87856 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECL_RS26315 87923..88678 - 756 Protein_106 IS481-like element ISKpn28 family transposase -
ECL_RS26325 89928..90209 - 282 Protein_108 helix-turn-helix domain-containing protein -
ECL_RS26330 90281..90439 + 159 WP_013087178.1 type I toxin-antitoxin system Hok family toxin -
ECL_RS26335 91315..91629 + 315 WP_044157807.1 hypothetical protein -
ECL_RS26340 91690..92151 + 462 WP_013087181.1 hypothetical protein -
ECL_RS26345 92239..92439 + 201 WP_013087182.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..199562 199562
- inside IScluster/Tn - - 85890..90209 4319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T24729 WP_000323025.1 NC_014107:87569-87856 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T24729 NC_014107:87569-87856 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT24729 WP_004196370.1 NC_014107:87330-87569 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT24729 NC_014107:87330-87569 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References