Detailed information of TA system TA024728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 72763..73502
• Replicon: plasmid pECL_A
• Accession: NC_014107
• Organism: Enterobacter cloacae subsp. cloacae ATCC 13047

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5E1AVR5
• Locus tag: ECL_RS26225
• Protein ID: WP_013087172.1
• Coordinates: 73017..73502 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: ECL_RS26220
• Protein ID: WP_012540086.1
• Coordinates: 72763..73029 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECL_RS26190	67993..68529	-	537	WP_013087166.1	GNAT family N-acetyltransferase	-
ECL_RS26195	68654..70405	-	1752	WP_162875198.1	arsenical pump-driving ATPase	-
ECL_RS26200	70432..70794	-	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
ECL_RS26205	70870..71415	-	546	WP_013087169.1	sigma-70 family RNA polymerase sigma factor	-
ECL_RS26210	71424..72137	-	714	WP_013087170.1	arsenical resistance protein ArsH	-
ECL_RS26215	72139..72462	-	324	WP_013087171.1	metalloregulator ArsR/SmtB family transcription factor	-
ECL_RS26220	72763..73029	+	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
ECL_RS26225	73017..73502	+	486	WP_013087172.1	GNAT family N-acetyltransferase	Toxin
ECL_RS26230	73921..75261	+	1341	WP_000589001.1	ISNCY family transposase	-
ECL_RS26235	75517..75939	-	423	WP_032072095.1	nickel resistance OB fold protein NcrY	-
ECL_RS26240	76101..77231	-	1131	WP_004196366.1	Ni(II)/Co(II) efflux transporter permease subunit NcrC	-
ECL_RS26245	77244..77513	-	270	WP_004196355.1	nickel-sensing transcriptional repressor NcrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..199562	199562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17810.62 Da
• Isoelectric Point: 9.8560

>T24728 WP_013087172.1 NC_014107:73017-73502 [Enterobacter cloacae subsp. cloacae ATCC 13047]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T24728 NC_014107:73017-73502 [Enterobacter cloacae subsp. cloacae ATCC 13047]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT24728 WP_012540086.1 NC_014107:72763-73029 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT24728 NC_014107:72763-73029 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A5E1AVR5

Antitoxin

• AlphaFold DB: A0A5E1AWX8