Detailed information of TA system TA024727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200086..200722
• Replicon: chromosome
• Accession: NC_014103
• Organism: Bacillus megaterium DSM 319

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: BMD_RS01105
• Protein ID: WP_013055004.1
• Coordinates: 200372..200722 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: BMD_RS01100
• Protein ID: WP_013055003.1
• Coordinates: 200086..200367 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMD_RS01075	195452..196423	+	972	WP_013081416.1	UV DNA damage repair endonuclease UvsE	-
BMD_RS01080	196432..197034	-	603	WP_013081417.1	rhomboid family intramembrane serine protease	-
BMD_RS01085	197099..197464	+	366	WP_013081418.1	holo-ACP synthase	-
BMD_RS01090	197523..198581	+	1059	WP_013081419.1	outer membrane lipoprotein carrier protein LolA	-
BMD_RS01095	198695..199885	+	1191	WP_013081420.1	alanine racemase	-
BMD_RS01100	200086..200367	+	282	WP_013055003.1	hypothetical protein	Antitoxin
BMD_RS01105	200372..200722	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BMD_RS01110	200882..201718	+	837	WP_013081421.1	STAS domain-containing protein	-
BMD_RS01115	201721..202077	+	357	WP_013081422.1	STAS domain-containing protein	-
BMD_RS01120	202081..202482	+	402	WP_013055007.1	anti-sigma regulatory factor	-
BMD_RS01125	202496..203506	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
BMD_RS01130	203566..203898	+	333	WP_013055009.1	anti-sigma factor antagonist	-
BMD_RS01135	203895..204380	+	486	WP_013055010.1	anti-sigma B factor RsbW	-
BMD_RS01140	204346..205140	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T24727 WP_013055004.1 NC_014103:200372-200722 [Bacillus megaterium DSM 319]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T24727 NC_014103:200372-200722 [Bacillus megaterium DSM 319]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT24727 WP_013055003.1 NC_014103:200086-200367 [Bacillus megaterium DSM 319]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT24727 NC_014103:200086-200367 [Bacillus megaterium DSM 319]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9