Detailed information of TA system TA024725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3265938..3266561
• Replicon: chromosome
• Accession: NC_014098
• Organism: Kyrpidia tusciae DSM 2912

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5WX25
• Locus tag: BTUS_RS15895
• Protein ID: WP_013077085.1
• Coordinates: 3265938..3266273 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BTUS_RS15900
• Protein ID: WP_013077086.1
• Coordinates: 3266277..3266561 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTUS_RS15865	3261527..3262051	-	525	WP_013077079.1	Uma2 family endonuclease	-
BTUS_RS15875	3262285..3262551	-	267	WP_013077080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BTUS_RS15880	3263019..3263270	+	252	WP_013077081.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BTUS_RS15885	3263267..3263704	+	438	WP_013077082.1	type II toxin-antitoxin system VapC family toxin	-
BTUS_RS17765	3264253..3264390	+	138	WP_083780338.1	2-oxo acid dehydrogenase subunit E2	-
BTUS_RS17205	3264459..3265169	+	711	WP_123809355.1	sugar ABC transporter permease	-
BTUS_RS15895	3265938..3266273	-	336	WP_013077085.1	endoribonuclease MazF	Toxin
BTUS_RS15900	3266277..3266561	-	285	WP_013077086.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BTUS_RS15905	3266695..3267210	-	516	WP_041306461.1	Uma2 family endonuclease	-
BTUS_RS17770	3267270..3267641	-	372	WP_083780261.1	hypothetical protein	-
BTUS_RS15915	3268111..3268341	-	231	WP_013077088.1	DUF2892 domain-containing protein	-
BTUS_RS18160	3268465..3270411	-	1947	WP_179943823.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-99)

Antitoxin

• Antitoxin PemI-like (18-92)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12219.04 Da
• Isoelectric Point: 5.6760

>T24725 WP_013077085.1 NC_014098:c3266273-3265938 [Kyrpidia tusciae DSM 2912]
MFVPERGDLVYVRFNPQAGHEQAGTRPAIVLSPKSFNAGTGFAVVCPITRQKKGYPFEVELPAGLSVEGVILTDQVKSLD
WRARQFQLKDHAPDEVVMDCLDLISTFLSVG

Nucleotide

• Length: 336 bp

>T24725 NC_014098:c3266273-3265938 [Kyrpidia tusciae DSM 2912]
ATGTTTGTACCGGAACGTGGAGATCTTGTATACGTGCGCTTCAATCCGCAAGCCGGTCACGAACAGGCGGGTACAAGGCC
AGCCATCGTATTGTCTCCAAAATCGTTTAACGCGGGGACGGGTTTTGCCGTTGTTTGCCCAATCACGCGCCAAAAAAAGG
GCTACCCATTCGAGGTTGAGTTGCCTGCAGGCCTAAGTGTCGAAGGTGTGATTCTTACAGATCAGGTAAAAAGTCTCGAT
TGGAGAGCCAGACAGTTTCAACTTAAAGACCACGCACCTGATGAAGTCGTCATGGACTGTTTAGATCTCATCAGTACCTT
TCTTTCTGTTGGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10588.20 Da
• Isoelectric Point: 7.0177

>AT24725 WP_013077086.1 NC_014098:c3266561-3266277 [Kyrpidia tusciae DSM 2912]
MMMEKGFTGKEESAVVIRTRVQKWGNSLAIRLPSAFAESISIQQGSEVELRLTNRTIIVVPRNRKPSLEDLLAKITPENR
HEDIDLGTEGKELL

Nucleotide

• Length: 285 bp

>AT24725 NC_014098:c3266561-3266277 [Kyrpidia tusciae DSM 2912]
ATGATGATGGAGAAGGGATTTACGGGTAAGGAGGAGAGCGCAGTGGTCATCAGGACGAGAGTGCAGAAGTGGGGCAACAG
CCTGGCCATACGCCTGCCCAGTGCGTTTGCCGAAAGTATTTCGATTCAACAGGGATCTGAGGTCGAACTCAGGCTGACAA
ACCGCACAATTATCGTGGTTCCGCGAAACCGAAAACCCTCTTTAGAGGACTTGCTGGCGAAGATCACCCCGGAAAACCGT
CACGAGGACATTGACTTGGGGACCGAAGGAAAGGAACTGCTTTAA

Structures

Toxin

• AlphaFold DB: D5WX25