Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1023373..1023956 Replicon chromosome
Accession NZ_CP098227
Organism Escherichia coli strain XJ6-3

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag KFZ54_RS04950 Protein ID WP_088376419.1
Coordinates 1023621..1023956 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KFZ54_RS04945 Protein ID WP_000581937.1
Coordinates 1023373..1023621 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFZ54_RS04935 (1019712) 1019712..1021013 + 1302 WP_000046821.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KFZ54_RS04940 (1021061) 1021061..1023295 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KFZ54_RS04945 (1023373) 1023373..1023621 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFZ54_RS04950 (1023621) 1023621..1023956 + 336 WP_088376419.1 endoribonuclease MazF Toxin
KFZ54_RS04955 (1024027) 1024027..1024818 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KFZ54_RS04960 (1025046) 1025046..1026683 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KFZ54_RS04965 (1026771) 1026771..1028069 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12069.99 Da        Isoelectric Point: 8.2618

>T247249 WP_088376419.1 NZ_CP098227:1023621-1023956 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCAPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT247249 WP_000581937.1 NZ_CP098227:1023373-1023621 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References