247220

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1222235..1223072 Replicon chromosome
Accession NZ_CP098223
Organism Escherichia coli strain Z0117EC0005

Toxin (Protein)


Gene name itaT Uniprot ID V0SED1
Locus tag NBY12_RS05835 Protein ID WP_000227787.1
Coordinates 1222235..1222777 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag NBY12_RS05840 Protein ID WP_001353405.1
Coordinates 1222761..1223072 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY12_RS05815 (1217765) 1217765..1218676 - 912 WP_000705864.1 2-dehydropantoate 2-reductase -
NBY12_RS05820 (1218844) 1218844..1219335 + 492 WP_001138904.1 nucleotide binding protein YajQ -
NBY12_RS05825 (1219463) 1219463..1220827 - 1365 WP_032302573.1 MFS transporter -
NBY12_RS05830 (1221244) 1221244..1222179 + 936 Protein_1126 tetratricopeptide repeat protein -
NBY12_RS05835 (1222235) 1222235..1222777 - 543 WP_000227787.1 GNAT family N-acetyltransferase Toxin
NBY12_RS05840 (1222761) 1222761..1223072 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
NBY12_RS05845 (1223257) 1223257..1224147 - 891 WP_000971336.1 heme o synthase -
NBY12_RS05850 (1224159) 1224159..1224488 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NBY12_RS05855 (1224488) 1224488..1225102 - 615 WP_000179807.1 cytochrome o ubiquinol oxidase subunit III -
NBY12_RS05860 (1225092) 1225092..1227083 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NBY12_RS05865 (1227105) 1227105..1228052 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19946.24 Da        Isoelectric Point: 8.8951

>T247220 WP_000227787.1 NZ_CP098223:c1222777-1222235 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT247220 WP_001353405.1 NZ_CP098223:c1223072-1222761 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GE43

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References