Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 685571..686147 Replicon chromosome
Accession NZ_CP098223
Organism Escherichia coli strain Z0117EC0005

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NBY12_RS03240 Protein ID WP_001297643.1
Coordinates 685571..685858 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NBY12_RS03245 Protein ID WP_000063156.1
Coordinates 685845..686147 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY12_RS03220 (681824) 681824..683104 - 1281 WP_001037380.1 DUF445 domain-containing protein -
NBY12_RS03225 (683145) 683145..684377 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
NBY12_RS03230 (684523) 684523..684618 - 96 Protein_615 dienelactone hydrolase family protein -
NBY12_RS03235 (684675) 684675..685372 + 698 Protein_616 IS1 family transposase -
NBY12_RS03240 (685571) 685571..685858 + 288 WP_001297643.1 BrnT family toxin Toxin
NBY12_RS03245 (685845) 685845..686147 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NBY12_RS03250 (686181) 686181..687137 - 957 WP_001297640.1 GTPase -
NBY12_RS03255 (687148) 687148..687351 - 204 WP_000467859.1 YbdD/YjiX family protein -
NBY12_RS03260 (687401) 687401..689551 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T247217 WP_001297643.1 NZ_CP098223:685571-685858 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT247217 WP_000063156.1 NZ_CP098223:685845-686147 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References