Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 453969..454466 Replicon chromosome
Accession NZ_CP098223
Organism Escherichia coli strain Z0117EC0005

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NBY12_RS02065 Protein ID WP_001173343.1
Coordinates 454293..454466 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NBY12_RS02060 Protein ID WP_000398619.1
Coordinates 453969..454265 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY12_RS02040 (450698) 450698..451417 - 720 WP_000611290.1 two-component system response regulator DcuR -
NBY12_RS02045 (451414) 451414..453045 - 1632 WP_032304335.1 sensor histidine kinase -
NBY12_RS02050 (453226) 453226..453456 + 231 WP_032304336.1 4Fe-4S mono-cluster protein YjdI -
NBY12_RS02055 (453468) 453468..453740 + 273 WP_000405650.1 GNAT family N-acetyltransferase -
NBY12_RS02060 (453969) 453969..454265 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NBY12_RS02065 (454293) 454293..454466 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NBY12_RS02070 (454585) 454585..456102 - 1518 WP_001775072.1 lysine--tRNA ligase -
NBY12_RS02075 (456339) 456339..457796 - 1458 WP_032304338.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T247216 WP_001173343.1 NZ_CP098223:454293-454466 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT247216 WP_000398619.1 NZ_CP098223:453969-454265 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References