Detailed information of TA system TA024720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-couple_hipB
• Location: 20288..20895
• Replicon: plasmid pRCB133
• Accession: NC_014035
• Organism: Rhodobacter capsulatus SB 1003

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RCAP_RS17635
• Protein ID: WP_044033738.1
• Coordinates: 20629..20895 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RCAP_RS17630
• Protein ID: WP_013069259.1
• Coordinates: 20288..20632 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RCAP_RS17600	15453..16223	+	771	WP_013069251.1	glucose-1-phosphate cytidylyltransferase	-
RCAP_RS19745	16208..16474	+	267	WP_013069252.1	hypothetical protein	-
RCAP_RS17605	16396..17232	+	837	WP_013069253.1	NAD-dependent epimerase/dehydratase family protein	-
RCAP_RS17610	17548..18006	-	459	WP_013069255.1	NUDIX hydrolase	-
RCAP_RS17615	18110..19000	-	891	WP_013069256.1	LysR family transcriptional regulator	-
RCAP_RS17620	19102..19425	+	324	WP_044033737.1	nuclear transport factor 2 family protein	-
RCAP_RS17625	19422..20201	+	780	WP_013069258.1	SDR family oxidoreductase	-
RCAP_RS17630	20288..20632	-	345	WP_013069259.1	helix-turn-helix transcriptional regulator	Antitoxin
RCAP_RS17635	20629..20895	-	267	WP_044033738.1	plasmid stabilization protein	Toxin
RCAP_RS17640	20986..21620	-	635	Protein_29	tyrosine-type recombinase/integrase	-
RCAP_RS17645	22237..22740	+	504	WP_013069263.1	metal-dependent hydrolase	-
RCAP_RS17650	22875..23123	+	249	WP_148214872.1	ATP-grasp domain-containing protein	-
RCAP_RS17655	23143..23601	+	459	WP_013069265.1	hypothetical protein	-
RCAP_RS17660	23613..24362	-	750	WP_013069266.1	hypothetical protein	-
RCAP_RS17665	24359..24985	-	627	WP_013069267.1	RNA ligase family protein	-
RCAP_RS17670	25027..25749	-	723	WP_023925871.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..132962	132962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (56-112)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 9452.09 Da
• Isoelectric Point: 11.1190

>T24720 WP_044033738.1 NC_014035:c20895-20629 [Rhodobacter capsulatus SB 1003]
MKQISTTKAAIKALRRMPSTTAALIRAKIEAYAQDPSAQANNVKPLKGREGIRLRVGDPRVIMDDQGNVLVLLDIGPRGG
VCDGKGLR

Nucleotide

• Length: 267 bp

>T24720 NC_014035:c20895-20629 [Rhodobacter capsulatus SB 1003]
ATGAAGCAGATCAGCACCACGAAAGCCGCGATCAAGGCCTTGCGGCGGATGCCCTCGACGACCGCTGCGCTGATCCGCGC
CAAGATCGAAGCTTACGCGCAAGACCCAAGCGCGCAGGCCAATAACGTGAAGCCCTTGAAAGGCCGGGAGGGTATTCGGC
TCAGGGTGGGGGACCCGCGGGTGATCATGGATGATCAGGGCAATGTTCTGGTCCTTCTCGATATCGGGCCGCGCGGCGGC
GTCTGCGACGGAAAGGGCTTGAGATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12303.85 Da
• Isoelectric Point: 4.3873

>AT24720 WP_013069259.1 NC_014035:c20632-20288 [Rhodobacter capsulatus SB 1003]
MTEMVTIPREEYDRLLAAAEDLADLQSYDRAKARLAAGEDELIPADYANRLLNGESALRVYRDLRGLTQAALAEKSGVNR
VTVAEIETGRKQGSIATLRALADALGVSLDDLAA

Nucleotide

• Length: 345 bp

>AT24720 NC_014035:c20632-20288 [Rhodobacter capsulatus SB 1003]
ATGACCGAGATGGTGACGATCCCGCGCGAAGAATATGACCGCCTGCTTGCTGCCGCGGAGGATCTGGCCGACCTGCAAAG
CTATGACCGCGCCAAGGCGCGGCTTGCGGCGGGAGAGGACGAGTTGATCCCGGCGGACTATGCGAACCGCCTTCTGAATG
GCGAAAGCGCCTTGCGCGTCTACCGCGATCTGCGCGGTCTGACGCAAGCTGCGCTGGCGGAAAAATCGGGCGTGAATCGG
GTCACCGTGGCCGAGATCGAGACCGGCCGAAAGCAGGGCTCGATCGCAACATTGCGCGCTCTGGCCGACGCGCTTGGCGT
GAGCTTGGATGATCTGGCGGCGTAA