Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1052573..1053227 Replicon chromosome
Accession NZ_CP098210
Organism Escherichia coli strain Z0117EC0051

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NBY17_RS04975 Protein ID WP_000244777.1
Coordinates 1052820..1053227 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NBY17_RS04970 Protein ID WP_000354046.1
Coordinates 1052573..1052839 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY17_RS04945 (1047742) 1047742..1048485 + 744 WP_000951964.1 SDR family oxidoreductase -
NBY17_RS04950 (1048542) 1048542..1049975 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
NBY17_RS04955 (1050020) 1050020..1050331 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
NBY17_RS04960 (1050495) 1050495..1051154 + 660 WP_000250274.1 hemolysin III family protein -
NBY17_RS04965 (1051350) 1051350..1052330 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
NBY17_RS04970 (1052573) 1052573..1052839 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NBY17_RS04975 (1052820) 1052820..1053227 + 408 WP_000244777.1 protein YgfX Toxin
NBY17_RS04980 (1053267) 1053267..1053788 - 522 WP_001055874.1 flavodoxin FldB -
NBY17_RS04985 (1053900) 1053900..1054796 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NBY17_RS04990 (1054821) 1054821..1055531 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NBY17_RS04995 (1055537) 1055537..1057270 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T247080 WP_000244777.1 NZ_CP098210:1052820-1053227 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T247080 NZ_CP098210:1052820-1053227 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT247080 WP_000354046.1 NZ_CP098210:1052573-1052839 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT247080 NZ_CP098210:1052573-1052839 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References