Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 55928..57551 Replicon plasmid pZ0117ECO054-1
Accession NZ_CP098207
Organism Escherichia coli strain Z0117EC0054

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NBY18_RS25160 Protein ID WP_000088046.1
Coordinates 55928..57253 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NBY18_RS25165 Protein ID WP_024136327.1
Coordinates 57258..57551 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY18_RS25130 (NBY18_25120) 52598..52897 - 300 WP_001572440.1 hypothetical protein -
NBY18_RS25135 (NBY18_25125) 52952..53188 - 237 Protein_67 type I restriction-modification system subunit M N-terminal domain-containing protein -
NBY18_RS25140 (NBY18_25130) 53642..54067 - 426 WP_001043843.1 hypothetical protein -
NBY18_RS25145 (NBY18_25135) 54321..55136 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NBY18_RS25150 (NBY18_25140) 55149..55559 - 411 WP_000985911.1 hypothetical protein -
NBY18_RS25155 (NBY18_25145) 55661..55867 - 207 WP_000134171.1 hypothetical protein -
NBY18_RS25160 (NBY18_25150) 55928..57253 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NBY18_RS25165 (NBY18_25155) 57258..57551 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NBY18_RS25170 (NBY18_25160) 58183..58575 - 393 WP_000341066.1 hypothetical protein -
NBY18_RS25175 (NBY18_25165) 58948..59289 + 342 WP_001151575.1 hypothetical protein -
NBY18_RS25180 (NBY18_25170) 59381..59572 + 192 WP_000398480.1 hypothetical protein -
NBY18_RS25185 (NBY18_25175) 59622..59903 + 282 WP_000077926.1 hypothetical protein -
NBY18_RS26550 60244..60375 - 132 WP_000374059.1 hypothetical protein -
NBY18_RS25190 (NBY18_25180) 60466..61707 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qacE / aadA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-15 - 1..173366 173366


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T247073 WP_000088046.1 NZ_CP098207:c57253-55928 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT247073 WP_024136327.1 NZ_CP098207:c57551-57258 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References