Detailed information of TA system TA024705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 201659..202295
• Replicon: chromosome
• Accession: NC_014019
• Organism: Bacillus megaterium QM B1551

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: BMQ_RS01135
• Protein ID: WP_013055004.1
• Coordinates: 201945..202295 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: BMQ_RS01130
• Protein ID: WP_013055003.1
• Coordinates: 201659..201940 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMQ_RS01105	197025..197996	+	972	WP_013054998.1	UV DNA damage repair endonuclease UvsE	-
BMQ_RS01110	198005..198607	-	603	WP_013054999.1	rhomboid family intramembrane serine protease	-
BMQ_RS01115	198672..199037	+	366	WP_013055000.1	holo-ACP synthase	-
BMQ_RS01120	199096..200154	+	1059	WP_013055001.1	outer membrane lipoprotein carrier protein LolA	-
BMQ_RS01125	200268..201458	+	1191	WP_013055002.1	alanine racemase	-
BMQ_RS01130	201659..201940	+	282	WP_013055003.1	hypothetical protein	Antitoxin
BMQ_RS01135	201945..202295	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BMQ_RS01140	202453..203289	+	837	WP_013055005.1	STAS domain-containing protein	-
BMQ_RS01145	203292..203648	+	357	WP_013055006.1	STAS domain-containing protein	-
BMQ_RS01150	203652..204053	+	402	WP_013055007.1	anti-sigma regulatory factor	-
BMQ_RS01155	204067..205077	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
BMQ_RS01160	205137..205469	+	333	WP_013055009.1	anti-sigma factor antagonist	-
BMQ_RS01165	205466..205951	+	486	WP_013055010.1	anti-sigma B factor RsbW	-
BMQ_RS01170	205917..206711	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T24705 WP_013055004.1 NC_014019:201945-202295 [Bacillus megaterium QM B1551]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T24705 NC_014019:201945-202295 [Bacillus megaterium QM B1551]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT24705 WP_013055003.1 NC_014019:201659-201940 [Bacillus megaterium QM B1551]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT24705 NC_014019:201659-201940 [Bacillus megaterium QM B1551]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9