Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4175781..4176357 Replicon chromosome
Accession NZ_CP098203
Organism Escherichia coli strain Z0117EC0055

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NBY19_RS20525 Protein ID WP_001297643.1
Coordinates 4176070..4176357 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NBY19_RS20520 Protein ID WP_000063156.1
Coordinates 4175781..4176083 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY19_RS20505 (4172377) 4172377..4174527 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NBY19_RS20510 (4174577) 4174577..4174780 + 204 WP_000467859.1 YbdD/YjiX family protein -
NBY19_RS20515 (4174791) 4174791..4175747 + 957 WP_001297640.1 GTPase -
NBY19_RS20520 (4175781) 4175781..4176083 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NBY19_RS20525 (4176070) 4176070..4176357 - 288 WP_001297643.1 BrnT family toxin Toxin
NBY19_RS20530 (4176556) 4176556..4177253 - 698 Protein_4026 IS1 family transposase -
NBY19_RS20535 (4177310) 4177310..4177405 + 96 Protein_4027 dienelactone hydrolase family protein -
NBY19_RS20540 (4177551) 4177551..4178783 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
NBY19_RS20545 (4178824) 4178824..4180104 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4176556..4176765 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T247045 WP_001297643.1 NZ_CP098203:c4176357-4176070 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT247045 WP_000063156.1 NZ_CP098203:c4176083-4175781 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References