Detailed information of TA system TA247004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1178370..1180059
• Replicon: chromosome
• Accession: NZ_CP098201
• Organism: Escherichia coli strain Z0117EC0056

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: NBY20_RS05755
• Protein ID: WP_000365809.1
• Coordinates: 1179139..1180059 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: NBY20_RS05750
• Protein ID: WP_001550661.1
• Coordinates: 1178370..1179152 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NBY20_RS05730 (1173679)	1173679..1173837	-	159	Protein_1102	integrase	-
NBY20_RS05735 (1173929)	1173929..1174031	-	103	Protein_1103	helix-turn-helix domain-containing protein	-
NBY20_RS05740 (1174028)	1174028..1174498	-	471	WP_000201185.1	hypothetical protein	-
NBY20_RS05745 (1174845)	1174845..1178054	-	3210	WP_001521910.1	RNA-directed DNA polymerase	-
NBY20_RS05750 (1178370)	1178370..1179152	+	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
NBY20_RS05755 (1179139)	1179139..1180059	+	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NBY20_RS05760 (1180493)	1180493..1181377	-	885	WP_001119037.1	LysR family transcriptional regulator	-
NBY20_RS05765 (1181642)	1181642..1182700	+	1059	WP_122987197.1	alpha/beta hydrolase	-
NBY20_RS05770 (1182842)	1182842..1183969	+	1128	WP_001550663.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T247004 WP_000365809.1 NZ_CP098201:1179139-1180059 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT247004 WP_001550661.1 NZ_CP098201:1178370-1179152 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: V0Z9M0