Detailed information of TA system TA024700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 45204..46006
• Replicon: plasmid pCHQ1
• Accession: NC_014007
• Organism: Sphingobium japonicum UT26S

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: D4Z8T4
• Locus tag: SJA_RS19820
• Protein ID: WP_013041607.1
• Coordinates: 45204..45731 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: D4Z8T5
• Locus tag: SJA_RS19825
• Protein ID: WP_013041608.1
• Coordinates: 45728..46006 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SJA_RS19785	40593..40826	-	234	WP_013041599.1	hypothetical protein	-
SJA_RS19790	40897..41925	-	1029	WP_013041600.1	hypothetical protein	-
SJA_RS19795	41942..42772	-	831	WP_013041601.1	hypothetical protein	-
SJA_RS19800	42924..43154	-	231	WP_013041602.1	hypothetical protein	-
SJA_RS19805	43322..43831	-	510	WP_013041603.1	GNAT family N-acetyltransferase	-
SJA_RS19810	43828..44118	-	291	WP_013041604.1	DUF1778 domain-containing protein	-
SJA_RS19815	44328..44711	-	384	WP_013041605.1	type II toxin-antitoxin system VapC family toxin	-
SJA_RS21625	44708..44965	-	258	WP_013041606.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
SJA_RS19820	45204..45731	-	528	WP_013041607.1	acetyltransferase	Toxin
SJA_RS19825	45728..46006	-	279	WP_013041608.1	DUF1778 domain-containing protein	Antitoxin
SJA_RS19830	46426..46737	-	312	WP_013041609.1	hypothetical protein	-
SJA_RS20940	46752..47513	-	762	WP_013041610.1	hypothetical protein	-
SJA_RS19840	47637..48620	-	984	WP_013041611.1	DUF1738 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..190974	190974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (11-88)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19192.94 Da
• Isoelectric Point: 6.8642

>T24700 WP_013041607.1 NC_014007:c45731-45204 [Sphingobium japonicum UT26S]
MTLPSWYEEAISKAHDRVSFDCGDDDLNIFLQRYARQSHELGGAKTFLAIDDADSKTVLGFYSIAPGSMDYADTPELVRR
GLARHEVPGFRLARLATDIRVQGKGLGGQLLGAIARRCIRVAAEVGGVMLIVDAKNERAAAWYASYGAVPLHDKPLTLIL
PFATLERELRAASQF

Nucleotide

• Length: 528 bp

>T24700 NC_014007:c45731-45204 [Sphingobium japonicum UT26S]
ATGACGCTTCCGTCATGGTATGAGGAAGCCATCTCGAAAGCGCACGACCGGGTCTCTTTCGATTGTGGCGACGACGACCT
CAATATTTTTTTGCAACGCTACGCGCGGCAGAGCCACGAACTAGGCGGGGCCAAGACCTTCCTCGCGATCGACGACGCCG
ACAGCAAGACGGTTCTCGGTTTCTACAGTATCGCGCCCGGCAGCATGGACTATGCCGATACACCCGAACTGGTGAGACGA
GGACTCGCCCGGCATGAGGTGCCCGGTTTCCGGCTCGCCCGGCTTGCGACGGATATTCGGGTGCAGGGGAAAGGCCTCGG
CGGTCAGCTCTTGGGTGCGATCGCGCGCAGATGCATTCGGGTTGCCGCGGAGGTTGGCGGGGTCATGCTTATCGTCGATG
CAAAAAACGAGCGCGCTGCCGCTTGGTATGCCAGCTATGGCGCCGTTCCGCTGCACGACAAACCGCTTACGCTTATATTG
CCCTTTGCAACGCTGGAACGCGAGTTGAGAGCGGCAAGCCAATTCTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10242.90 Da
• Isoelectric Point: 10.5767

>AT24700 WP_013041608.1 NC_014007:c46006-45728 [Sphingobium japonicum UT26S]
MPRAVVEDNRRMSLRIRPEQKALLARAAALKHTDLTTFVTRSALREAESVIEEAERTKLSERDSLLVLDLLENPPPANAK
LRAAIAAMPKQA

Nucleotide

• Length: 279 bp

>AT24700 NC_014007:c46006-45728 [Sphingobium japonicum UT26S]
ATGCCGCGAGCTGTGGTGGAAGATAACAGGCGCATGAGTCTGCGGATTCGACCGGAGCAAAAGGCTTTGCTCGCCCGGGC
GGCTGCGCTCAAGCATACCGACCTAACCACCTTTGTCACACGGTCGGCCCTCCGTGAGGCCGAGAGCGTGATCGAGGAGG
CGGAGCGGACGAAGCTGAGCGAGAGAGACAGCCTGCTCGTTCTCGATCTGCTCGAAAACCCGCCGCCGGCGAATGCGAAA
TTACGTGCCGCCATCGCCGCGATGCCAAAGCAGGCATGA

Structures

Toxin

• AlphaFold DB: D4Z8T4

Antitoxin

• AlphaFold DB: D4Z8T5