Detailed information of TA system TA024699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 23504..24038
• Replicon: plasmid pCHQ1
• Accession: NC_014007
• Organism: Sphingobium japonicum UT26S

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W1S6F4
• Locus tag: SJA_RS19670
• Protein ID: WP_004212931.1
• Coordinates: 23504..23785 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W1S0F5
• Locus tag: SJA_RS19675
• Protein ID: WP_004212930.1
• Coordinates: 23772..24038 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SJA_RS22270	18981..19088	+	108	Protein_23	IS6 family transposase	-
SJA_RS19640	19142..19906	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
SJA_RS19645	19935..20990	+	1056	WP_013041570.1	efflux transporter outer membrane subunit	-
SJA_RS19650	21122..21508	+	387	WP_148225626.1	hypothetical protein	-
SJA_RS19655	21523..21918	+	396	WP_013041572.1	CopG family transcriptional regulator	-
SJA_RS19660	21915..22877	+	963	WP_013041573.1	tyrosine-type recombinase/integrase	-
SJA_RS19665	22874..23494	-	621	WP_013041574.1	histidine phosphatase family protein	-
SJA_RS19670	23504..23785	-	282	WP_004212931.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SJA_RS19675	23772..24038	-	267	WP_004212930.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SJA_RS19680	24469..25110	+	642	WP_013041576.1	transcription elongation protein SprT	-
SJA_RS19685	25186..25839	+	654	WP_013041577.1	AAA family ATPase	-
SJA_RS19690	25836..26150	+	315	WP_013041578.1	hypothetical protein	-
SJA_RS19695	26232..26696	+	465	WP_031309919.1	GNAT family N-acetyltransferase	-
SJA_RS19700	26786..27436	-	651	Protein_36	replication initiator protein A	-
SJA_RS19705	27485..28249	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
SJA_RS22670	28221..28304	-	84	Protein_38	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..190974	190974
	-	inside	IScluster/Tn	-	-	12628..19906	7278
	-	flank	IS/Tn	-	-	27485..28249	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-85)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10667.04 Da
• Isoelectric Point: 8.0689

>T24699 WP_004212931.1 NC_014007:c23785-23504 [Sphingobium japonicum UT26S]
MRTIDRSTRFKRDYKRESKGRHRATLDADLLPVLAALATDTPLDPKYRDHDLSGDWAGYRDCHVKPDLVLIYAKPDDETL
RLARLGSHSEVFG

Nucleotide

• Length: 282 bp

>T24699 NC_014007:c23785-23504 [Sphingobium japonicum UT26S]
ATGCGGACGATTGATCGTTCGACCCGGTTCAAGCGCGACTACAAGCGGGAATCAAAGGGCCGGCATCGAGCGACCCTCGA
TGCCGATCTTCTGCCCGTCCTGGCCGCGCTCGCGACCGACACGCCGCTTGATCCCAAATACCGCGACCATGATCTGAGCG
GCGATTGGGCGGGCTACCGCGATTGCCACGTCAAGCCCGATCTGGTGCTGATCTACGCCAAGCCCGACGACGAAACGCTG
CGCCTCGCGCGCCTTGGCTCGCATAGCGAGGTATTCGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9448.88 Da
• Isoelectric Point: 5.8213

>AT24699 WP_004212930.1 NC_014007:c24038-23772 [Sphingobium japonicum UT26S]
MAVSDTYVRARIDNTTKERATAALGAMGLSISDAIRLLMLRIADERRLPFEVKVPNAATREAMAELAAGKGTKFASVDAL
MADLHADD

Nucleotide

• Length: 267 bp

>AT24699 NC_014007:c24038-23772 [Sphingobium japonicum UT26S]
ATGGCTGTTTCCGACACTTATGTCCGCGCCAGGATCGACAATACAACGAAGGAACGCGCCACCGCCGCGCTGGGCGCGAT
GGGCCTTTCGATCTCCGACGCTATCCGGCTGCTGATGCTGCGTATCGCCGACGAACGGCGCTTGCCTTTCGAGGTCAAGG
TTCCGAACGCCGCGACACGCGAGGCAATGGCAGAGCTGGCGGCGGGGAAGGGGACCAAGTTCGCCAGCGTCGATGCGCTG
ATGGCGGACCTCCATGCGGACGATTGA

Structures

Toxin

• AlphaFold DB: W1S6F4

Antitoxin

• AlphaFold DB: W1S0F5