Detailed information of TA system TA024698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 15555..16252
• Replicon: plasmid pCHQ1
• Accession: NC_014007
• Organism: Sphingobium japonicum UT26S

Toxin (Protein)

• Gene name: tad
• Uniprot ID: T0J3S2
• Locus tag: SJA_RS19610
• Protein ID: WP_013041565.1
• Coordinates: 15555..15929 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: T0G937
• Locus tag: SJA_RS19615
• Protein ID: WP_006964370.1
• Coordinates: 15926..16252 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SJA_RS22720	11279..11371	+	93	Protein_11	IS5/IS1182 family transposase	-
SJA_RS19590	11833..12165	-	333	WP_007682049.1	BrnA antitoxin family protein	-
SJA_RS19595	12146..12454	-	309	WP_007682056.1	BrnT family toxin	-
SJA_RS19600	12628..13392	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
SJA_RS19605	13589..14860	+	1272	Protein_15	Tn3 family transposase	-
SJA_RS21575	14891..15133	-	243	Protein_16	hypothetical protein	-
SJA_RS19610	15555..15929	+	375	WP_013041565.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SJA_RS19615	15926..16252	+	327	WP_006964370.1	helix-turn-helix domain-containing protein	Antitoxin
SJA_RS19620	16252..16494	+	243	WP_013041566.1	hypothetical protein	-
SJA_RS19625	16487..16786	+	300	WP_013041567.1	hypothetical protein	-
SJA_RS19630	16807..17919	-	1113	WP_013041568.1	muramidase	-
SJA_RS19635	17922..18917	-	996	Protein_22	P-type conjugative transfer protein TrbL	-
SJA_RS22270	18981..19088	+	108	Protein_23	IS6 family transposase	-
SJA_RS19640	19142..19906	-	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
SJA_RS19645	19935..20990	+	1056	WP_013041570.1	efflux transporter outer membrane subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..190974	190974
	-	inside	IScluster/Tn	-	-	12628..19906	7278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (15-94)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13960.67 Da
• Isoelectric Point: 9.4885

>T24698 WP_013041565.1 NC_014007:15555-15929 [Sphingobium japonicum UT26S]
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDHQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKSAEEHHRQHQTPRGTA

Nucleotide

• Length: 375 bp

>T24698 NC_014007:15555-15929 [Sphingobium japonicum UT26S]
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTTGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATCATAGACGACCACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTCGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAATCCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11792.57 Da
• Isoelectric Point: 7.1681

>AT24698 WP_006964370.1 NC_014007:15926-16252 [Sphingobium japonicum UT26S]
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA

Nucleotide

• Length: 327 bp

>AT24698 NC_014007:15926-16252 [Sphingobium japonicum UT26S]
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGACCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTGGCCGAGGCCATGCAGGAGCGGAAGTTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACCCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTAGCC
ATGCTCACCCGGCTTGGCTGCAAGGTCGATATTGTCGTGCGCCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0J7XHG4

Antitoxin

• AlphaFold DB: A0A0J8A803