Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3041621..3042405 Replicon chromosome
Accession NZ_CP098197
Organism Escherichia coli strain Z0117EC0062

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag NBY21_RS14705 Protein ID WP_000613626.1
Coordinates 3041911..3042405 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag NBY21_RS14700 Protein ID WP_001110447.1
Coordinates 3041621..3041914 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY21_RS14690 (3036771) 3036771..3037730 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
NBY21_RS14695 (3038303) 3038303..3041488 + 3186 WP_001550951.1 ribonuclease E -
NBY21_RS14700 (3041621) 3041621..3041914 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
NBY21_RS14705 (3041911) 3041911..3042405 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
NBY21_RS14710 (3042500) 3042500..3043453 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
NBY21_RS14715 (3043465) 3043465..3045108 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
NBY21_RS14720 (3045174) 3045174..3046115 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
NBY21_RS14725 (3046115) 3046115..3047212 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T246976 WP_000613626.1 NZ_CP098197:3041911-3042405 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT246976 WP_001110447.1 NZ_CP098197:3041621-3041914 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References