Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 11833..12454 Replicon plasmid pCHQ1
Accession NC_014007
Organism Sphingobium japonicum UT26S

Toxin (Protein)


Gene name shpA Uniprot ID M4S8P5
Locus tag SJA_RS19595 Protein ID WP_007682056.1
Coordinates 12146..12454 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID T0GLH9
Locus tag SJA_RS19590 Protein ID WP_007682049.1
Coordinates 11833..12165 (-) Length 111 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SJA_RS19580 9819..10370 + 552 WP_041386225.1 recombinase family protein -
SJA_RS19585 10367..11155 + 789 WP_013039098.1 SDR family NAD(P)-dependent oxidoreductase -
SJA_RS22720 11279..11371 + 93 Protein_11 IS5/IS1182 family transposase -
SJA_RS19590 11833..12165 - 333 WP_007682049.1 BrnA antitoxin family protein Antitoxin
SJA_RS19595 12146..12454 - 309 WP_007682056.1 BrnT family toxin Toxin
SJA_RS19600 12628..13392 + 765 WP_001389365.1 IS6-like element IS6100 family transposase -
SJA_RS19605 13589..14860 + 1272 Protein_15 Tn3 family transposase -
SJA_RS21575 14891..15133 - 243 Protein_16 hypothetical protein -
SJA_RS19610 15555..15929 + 375 WP_013041565.1 type II toxin-antitoxin system RelE/ParE family toxin -
SJA_RS19615 15926..16252 + 327 WP_006964370.1 helix-turn-helix domain-containing protein -
SJA_RS19620 16252..16494 + 243 WP_013041566.1 hypothetical protein -
SJA_RS19625 16487..16786 + 300 WP_013041567.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..190974 190974
- inside IScluster/Tn - - 12628..19906 7278


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(8-93)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(19-99)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11771.19 Da        Isoelectric Point: 6.2230

>T24697 WP_007682056.1 NC_014007:c12454-12146 [Sphingobium japonicum UT26S]
MLSMRFSWHPAKAESNLRKHGISFETAASAFADPFALSVHDRIEGGEQRWQTLGLVEGHLLLLVAHTVAEDDDDGEYVEI
IHIISARKADRKERRRYEEERR

Download         Length: 309 bp

>T24697 NC_014007:c12454-12146 [Sphingobium japonicum UT26S]
ATGTTATCCATGCGCTTTTCCTGGCACCCAGCCAAAGCCGAGAGCAATCTGAGAAAGCATGGCATCAGTTTCGAGACGGC
CGCCAGCGCCTTCGCAGACCCCTTTGCGCTTAGCGTGCATGATCGGATCGAAGGCGGCGAGCAACGATGGCAGACCTTGG
GCCTGGTCGAAGGACATTTACTCCTGCTGGTGGCCCACACCGTCGCTGAGGATGACGACGACGGCGAATATGTGGAGATC
ATCCATATCATTTCGGCCCGAAAAGCCGATCGAAAGGAAAGACGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin


Download         Length: 111 a.a.        Molecular weight: 12476.26 Da        Isoelectric Point: 9.8217

>AT24697 WP_007682049.1 NC_014007:c12165-11833 [Sphingobium japonicum UT26S]
MKKKDADTVRFQLDPGNLPPLTEAQKAELDALQAMPDSGIDYSDAPTLTEDFWKTAERGRFYKPIKQQVTARLDADVLAW
LKSQGKGYQARMNAILRREMLAAAKERRHA

Download         Length: 333 bp

>AT24697 NC_014007:c12165-11833 [Sphingobium japonicum UT26S]
ATGAAGAAGAAAGACGCTGATACCGTCCGCTTCCAGCTCGATCCGGGCAATCTGCCCCCCTTGACCGAGGCACAGAAGGC
CGAGCTGGATGCCCTGCAGGCGATGCCGGATAGCGGTATCGACTATAGTGATGCCCCGACGCTGACAGAGGATTTCTGGA
AGACCGCCGAGCGTGGACGGTTCTATAAGCCGATCAAGCAGCAGGTAACGGCGCGGCTCGACGCCGATGTGCTTGCTTGG
CTCAAATCCCAGGGCAAGGGCTATCAGGCTCGCATGAACGCAATCCTTCGGCGCGAGATGTTGGCGGCTGCGAAGGAGCG
CCGACACGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A126RWR1

Antitoxin

Source ID Structure
AlphaFold DB T0GLH9

References