Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1046035..1046689 Replicon chromosome
Accession NZ_CP098197
Organism Escherichia coli strain Z0117EC0062

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NBY21_RS05155 Protein ID WP_000244777.1
Coordinates 1046282..1046689 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NBY21_RS05150 Protein ID WP_000354046.1
Coordinates 1046035..1046301 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY21_RS05125 (1041204) 1041204..1041947 + 744 WP_000951964.1 SDR family oxidoreductase -
NBY21_RS05130 (1042004) 1042004..1043437 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
NBY21_RS05135 (1043482) 1043482..1043793 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
NBY21_RS05140 (1043957) 1043957..1044616 + 660 WP_000250274.1 hemolysin III family protein -
NBY21_RS05145 (1044812) 1044812..1045792 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
NBY21_RS05150 (1046035) 1046035..1046301 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NBY21_RS05155 (1046282) 1046282..1046689 + 408 WP_000244777.1 protein YgfX Toxin
NBY21_RS05160 (1046729) 1046729..1047250 - 522 WP_001055874.1 flavodoxin FldB -
NBY21_RS05165 (1047362) 1047362..1048258 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NBY21_RS05170 (1048283) 1048283..1048993 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NBY21_RS05175 (1048999) 1048999..1050732 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T246967 WP_000244777.1 NZ_CP098197:1046282-1046689 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT246967 WP_000354046.1 NZ_CP098197:1046035-1046301 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References