Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2971461..2972038 Replicon chromosome
Accession NC_014006
Organism Sphingobium japonicum UT26S

Toxin (Protein)


Gene name mazF Uniprot ID D4Z5D7
Locus tag SJA_RS14155 Protein ID WP_013041111.1
Coordinates 2971461..2971796 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D4Z5D8
Locus tag SJA_RS14160 Protein ID WP_013041112.1
Coordinates 2971796..2972038 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SJA_RS22630 2967426..2967572 - 147 WP_167532622.1 hypothetical protein -
SJA_RS14130 2967845..2968533 - 689 Protein_2829 pentapeptide repeat-containing protein -
SJA_RS14135 2968627..2969277 - 651 WP_013041105.1 lytic transglycosylase domain-containing protein -
SJA_RS14140 2969265..2969837 - 573 WP_013041106.1 signal peptidase I -
SJA_RS14145 2969834..2970091 - 258 WP_013041107.1 helix-turn-helix domain-containing protein -
SJA_RS14150 2970267..2970587 - 321 WP_013041108.1 DUF736 domain-containing protein -
SJA_RS14155 2971461..2971796 - 336 WP_013041111.1 endoribonuclease MazF Toxin
SJA_RS14160 2971796..2972038 - 243 WP_013041112.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
SJA_RS14165 2972163..2972372 - 210 WP_013041113.1 hypothetical protein -
SJA_RS14170 2972553..2974427 - 1875 WP_013041114.1 ParB N-terminal domain-containing protein -
SJA_RS14175 2974544..2974945 - 402 WP_013041115.1 DUF2958 domain-containing protein -
SJA_RS14180 2974942..2975256 - 315 WP_148225568.1 antirestriction protein ArdC -
SJA_RS14185 2975253..2976155 - 903 WP_013039035.1 DUF1738 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2934452..3030065 95613


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(6-48)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12025.02 Da        Isoelectric Point: 9.5826

>T24696 WP_013041111.1 NC_014006:c2971796-2971461 [Sphingobium japonicum UT26S]
MASRYIPDTGDIVWLQFDPQAGHEQAGHRPALVLSPAAYNKLRGMMICCPMTSQIKGYPFEVTVSQAPPSIVLSDQIKSL
DWKARGAKKKGSVSTAVIDEVRAKIGTLLGL

Download         Length: 336 bp

>T24696 NC_014006:c2971796-2971461 [Sphingobium japonicum UT26S]
ATGGCCAGTCGCTATATTCCGGACACTGGCGATATTGTGTGGCTGCAATTCGATCCCCAGGCCGGACATGAGCAAGCGGG
GCATCGTCCTGCGCTCGTCCTCTCACCTGCGGCCTACAACAAGCTGCGGGGCATGATGATTTGCTGCCCGATGACGAGCC
AGATCAAGGGCTATCCTTTCGAAGTGACCGTGTCCCAGGCACCGCCCAGCATCGTGCTGTCCGATCAGATCAAGAGCCTC
GATTGGAAGGCGCGGGGAGCGAAGAAAAAGGGCTCCGTATCAACGGCCGTGATCGATGAGGTGCGCGCCAAGATAGGCAC
ACTTCTGGGTCTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8624.08 Da        Isoelectric Point: 4.6653

>AT24696 WP_013041112.1 NC_014006:c2972038-2971796 [Sphingobium japonicum UT26S]
MGIQVKKWGNSAAIRIPSAIMAAAALNVGQEVEVRQEGGRLIIEPVLKPVYTLDALLADMKPETFHEIVDFGPPVGNEVL

Download         Length: 243 bp

>AT24696 NC_014006:c2972038-2971796 [Sphingobium japonicum UT26S]
ATGGGTATTCAGGTAAAGAAGTGGGGCAATAGCGCGGCAATCCGGATTCCCTCCGCCATCATGGCGGCGGCCGCGCTGAA
TGTCGGTCAGGAGGTCGAAGTCCGCCAGGAAGGCGGTCGGCTCATCATCGAGCCCGTCCTGAAGCCTGTCTATACGCTCG
ACGCATTGCTTGCGGACATGAAGCCGGAGACGTTCCACGAGATTGTCGATTTTGGTCCCCCGGTCGGCAATGAAGTGCTT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4Z5D7

Antitoxin

Source ID Structure
AlphaFold DB A0A848HW50

References