Detailed information of TA system TA024695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2936216..2936781
• Replicon: chromosome
• Accession: NC_014006
• Organism: Sphingobium japonicum UT26S

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D4Z593
• Locus tag: SJA_RS13985
• Protein ID: WP_013041067.1
• Coordinates: 2936410..2936781 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SJA_RS21330
• Protein ID: WP_013041066.1
• Coordinates: 2936216..2936413 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SJA_RS13955	2931534..2932427	-	894	WP_013041062.1	N-formylglutamate amidohydrolase	-
SJA_RS13960	2932578..2932955	+	378	WP_007687442.1	response regulator	-
SJA_RS13965	2932959..2934257	-	1299	WP_193377939.1	dicarboxylate/amino acid:cation symporter	-
SJA_RS13975	2934785..2935027	-	243	WP_065846227.1	hypothetical protein	-
SJA_RS13980	2935029..2936066	+	1038	WP_013041065.1	site-specific integrase	-
SJA_RS21330	2936216..2936413	+	198	WP_013041066.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SJA_RS13985	2936410..2936781	+	372	WP_013041067.1	type II toxin-antitoxin system VapC family toxin	Toxin
SJA_RS13990	2937186..2939039	-	1854	WP_013041068.1	HipA domain-containing protein	-
SJA_RS22015	2939167..2939319	-	153	WP_139111437.1	IS110 family transposase	-
SJA_RS13995	2939553..2939894	+	342	WP_049775108.1	tyrosine-type recombinase/integrase	-
SJA_RS14000	2940093..2940476	-	384	WP_161601887.1	type II toxin-antitoxin system death-on-curing family toxin	-
SJA_RS14005	2940865..2941296	+	432	WP_013041074.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2934452..3030065	95613

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13809.98 Da
• Isoelectric Point: 6.4909

>T24695 WP_013041067.1 NC_014006:2936410-2936781 [Sphingobium japonicum UT26S]
MILVDTSVWIDHLRHDDPVLSQSLSKRQVLSHPFIIGELALGSLRQRDLILEALRGLPSVIVAHDEEVHAFIDLHRLFGI
GIGYIDAHLLAATLLTPDVQFWTRDKRLRAAALRLGVDANLDH

Nucleotide

• Length: 372 bp

>T24695 NC_014006:2936410-2936781 [Sphingobium japonicum UT26S]
ATGATCTTGGTCGACACTTCGGTCTGGATTGATCATTTGCGTCATGATGATCCCGTCCTTTCCCAATCGCTCAGCAAACG
GCAGGTTCTCTCCCATCCTTTCATCATCGGGGAGTTGGCACTGGGCAGTCTTCGGCAAAGAGACCTCATTCTCGAAGCGC
TACGTGGATTGCCTTCAGTTATTGTCGCCCACGACGAGGAAGTTCATGCCTTTATAGACCTTCATAGGCTCTTCGGTATT
GGCATCGGCTACATCGATGCACACCTGCTGGCAGCAACCCTTCTAACGCCTGACGTGCAATTCTGGACACGTGACAAGCG
GTTGCGGGCCGCGGCCTTGCGTCTGGGCGTGGATGCGAATCTGGATCACTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7069.07 Da
• Isoelectric Point: 8.9168

>AT24695 WP_013041066.1 NC_014006:2936216-2936413 [Sphingobium japonicum UT26S]
MRTTVALDDELVAKAQAFTGLTEKSSLVREALKALIERESSARLVRLGGTEPHGEAAPRRRPDPV

Nucleotide

• Length: 198 bp

>AT24695 NC_014006:2936216-2936413 [Sphingobium japonicum UT26S]
ATGCGGACTACAGTCGCTCTCGATGATGAACTTGTCGCGAAGGCACAGGCTTTCACCGGCCTGACCGAAAAATCGTCACT
CGTTCGCGAAGCGCTGAAAGCCCTCATAGAAAGGGAGAGCTCAGCGCGGCTTGTCCGCCTTGGTGGCACTGAGCCTCATG
GTGAAGCCGCGCCACGCCGACGCCCCGATCCTGTATGA

Structures

Toxin

• AlphaFold DB: D4Z593