Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 449532..450332 Replicon chromosome
Accession NZ_CP098195
Organism Escherichia coli strain Z0117EC0098

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NBY23_RS02175 Protein ID WP_000342449.1
Coordinates 449805..450332 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NBY23_RS02170 Protein ID WP_001277108.1
Coordinates 449532..449798 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY23_RS02150 (445190) 445190..445858 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NBY23_RS02155 (445851) 445851..446909 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NBY23_RS02160 (447154) 447154..448008 + 855 WP_063100092.1 RNA polymerase sigma factor RpoH -
NBY23_RS02165 (448279) 448279..449382 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NBY23_RS02170 (449532) 449532..449798 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NBY23_RS02175 (449805) 449805..450332 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NBY23_RS02180 (450329) 450329..450712 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
NBY23_RS02185 (451136) 451136..452245 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NBY23_RS02190 (452293) 452293..453219 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NBY23_RS02195 (453216) 453216..454493 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
NBY23_RS02200 (454490) 454490..455257 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T246938 WP_000342449.1 NZ_CP098195:449805-450332 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT246938 WP_001277108.1 NZ_CP098195:449532-449798 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References