Detailed information of TA system TA024693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 11496..12130
• Replicon: plasmid pUT1
• Accession: NC_014005
• Organism: Sphingobium japonicum UT26S

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: T0GCG3
• Locus tag: SJA_RS20610
• Protein ID: WP_007682494.1
• Coordinates: 11741..12130 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: T0GE84
• Locus tag: SJA_RS20605
• Protein ID: WP_007682496.1
• Coordinates: 11496..11744 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SJA_RS20590	7799..8563	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
SJA_RS20595	8657..9931	+	1275	WP_013038664.1	efflux RND transporter permease subunit	-
SJA_RS20600	9924..11327	+	1404	WP_013038665.1	efflux transporter outer membrane subunit	-
SJA_RS20605	11496..11744	+	249	WP_007682496.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SJA_RS20610	11741..12130	+	390	WP_007682494.1	type II toxin-antitoxin system VapC family toxin	Toxin
SJA_RS20615	12130..12435	+	306	WP_007682492.1	hypothetical protein	-
SJA_RS20620	12432..12830	+	399	WP_007682491.1	hypothetical protein	-
SJA_RS20625	12830..13792	+	963	WP_007682488.1	tyrosine-type recombinase/integrase	-
SJA_RS20630	13856..14953	+	1098	WP_007683624.1	IS630 family transposase	-
SJA_RS21730	14999..15322	-	324	Protein_20	transposase	-
SJA_RS20635	15404..16267	-	864	WP_004211466.1	IS21-like element helper ATPase IstB	-
SJA_RS21735	16264..16816	-	553	Protein_22	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..31776	31776
	-	flank	IS/Tn	-	-	7799..8563	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-123)

Antitoxin

• Antitoxin VapB-like (1-81)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14083.02 Da
• Isoelectric Point: 4.5934

>T24693 WP_007682494.1 NC_014005:11741-12130 [Sphingobium japonicum UT26S]
MILDTSALVAILYREPEAATFAQAIHDADACRISVASYVELSMVIERQLGPEGMRQTEAFFRRAGITIEPVTLDHGELAR
QAFLDFGKGRHKAGLNFGDCFSYALAKATDEPLLFKGNDFALTDIEAAA

Nucleotide

• Length: 390 bp

>T24693 NC_014005:11741-12130 [Sphingobium japonicum UT26S]
ATGATCCTCGACACCTCGGCCCTGGTCGCGATCCTCTATCGCGAACCCGAAGCGGCGACCTTCGCACAAGCCATCCATGA
TGCCGATGCCTGCCGCATCAGCGTCGCCAGCTATGTCGAGCTGTCGATGGTCATCGAACGCCAGCTCGGTCCCGAAGGGA
TGCGCCAGACCGAAGCCTTCTTTCGCCGCGCCGGCATCACGATCGAACCGGTGACGCTCGATCATGGCGAACTGGCGCGA
CAGGCGTTTCTCGATTTCGGCAAGGGCCGCCACAAAGCGGGCCTGAACTTTGGCGACTGCTTTTCCTATGCGCTGGCCAA
GGCGACCGACGAGCCGCTGTTGTTCAAGGGCAATGATTTTGCCCTGACGGACATAGAGGCGGCGGCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9203.47 Da
• Isoelectric Point: 9.7320

>AT24693 WP_007682496.1 NC_014005:11496-11744 [Sphingobium japonicum UT26S]
MSLNVKDPEAHRLAQAIAQATGQSMTRVVTEALRERFARIERQKSKASVAELLTIADRAATHVKRPYVDHAELFYDDNGL
PK

Nucleotide

• Length: 249 bp

>AT24693 NC_014005:11496-11744 [Sphingobium japonicum UT26S]
ATGAGCCTCAATGTTAAAGACCCTGAAGCCCATCGGCTTGCGCAGGCCATCGCCCAAGCGACCGGCCAGAGCATGACCCG
CGTGGTGACGGAGGCGCTGCGCGAGCGCTTCGCCAGGATCGAGCGTCAGAAAAGCAAGGCGAGCGTCGCGGAACTGCTAA
CGATCGCCGATCGCGCCGCCACGCATGTGAAGCGCCCCTATGTCGATCATGCCGAACTGTTTTATGACGATAATGGCCTG
CCGAAATGA

Structures

Toxin

• AlphaFold DB: T0GCG3

Antitoxin

• AlphaFold DB: T0GE84