Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 331689..332489 Replicon chromosome
Accession NZ_CP098192
Organism Escherichia coli strain Z0117EC0116

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NBY24_RS01545 Protein ID WP_000342449.1
Coordinates 331962..332489 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NBY24_RS01540 Protein ID WP_001277108.1
Coordinates 331689..331955 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY24_RS01520 (327346) 327346..328014 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NBY24_RS01525 (328007) 328007..329065 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NBY24_RS01530 (329310) 329310..330164 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NBY24_RS01535 (330436) 330436..331539 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NBY24_RS01540 (331689) 331689..331955 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NBY24_RS01545 (331962) 331962..332489 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NBY24_RS01550 (332486) 332486..332869 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
NBY24_RS01555 (333293) 333293..334402 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NBY24_RS01560 (334450) 334450..335376 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NBY24_RS01565 (335373) 335373..336650 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
NBY24_RS01570 (336647) 336647..337414 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T246906 WP_000342449.1 NZ_CP098192:331962-332489 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT246906 WP_001277108.1 NZ_CP098192:331689-331955 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References