Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4174350..4175004 Replicon chromosome
Accession NZ_CP098187
Organism Escherichia coli strain Z0117EC0117

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NBY25_RS20230 Protein ID WP_000244781.1
Coordinates 4174350..4174757 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NBY25_RS20235 Protein ID WP_000354046.1
Coordinates 4174738..4175004 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY25_RS20210 (4170307) 4170307..4172040 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
NBY25_RS20215 (4172046) 4172046..4172756 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NBY25_RS20220 (4172781) 4172781..4173677 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NBY25_RS20225 (4173789) 4173789..4174310 + 522 WP_001055874.1 flavodoxin FldB -
NBY25_RS20230 (4174350) 4174350..4174757 - 408 WP_000244781.1 protein YgfX Toxin
NBY25_RS20235 (4174738) 4174738..4175004 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NBY25_RS20240 (4175247) 4175247..4176227 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
NBY25_RS20245 (4176304) 4176304..4176963 - 660 WP_000250274.1 hemolysin III family protein -
NBY25_RS20250 (4177127) 4177127..4177438 - 312 WP_001182948.1 N(4)-acetylcytidine aminohydrolase -
NBY25_RS20255 (4177483) 4177483..4178916 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T246897 WP_000244781.1 NZ_CP098187:c4174757-4174350 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT246897 WP_000354046.1 NZ_CP098187:c4175004-4174738 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References