Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 114685..116308 Replicon plasmid pZ0117ECO0133-1
Accession NZ_CP098184
Organism Escherichia coli strain Z0117EC0133

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag NBY27_RS23635 Protein ID WP_086722013.1
Coordinates 114983..116308 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NBY27_RS23630 Protein ID WP_024136327.1
Coordinates 114685..114978 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY27_RS23605 (NBY27_23605) 110568..111809 + 1242 WP_001053338.1 VWA domain-containing protein -
NBY27_RS25050 111900..112031 + 132 WP_000374059.1 hypothetical protein -
NBY27_RS23610 (NBY27_23610) 112372..112653 - 282 WP_000077926.1 hypothetical protein -
NBY27_RS23615 (NBY27_23615) 112703..112894 - 192 WP_000398480.1 hypothetical protein -
NBY27_RS23620 (NBY27_23620) 112986..113327 - 342 WP_001151575.1 hypothetical protein -
NBY27_RS23625 (NBY27_23625) 113860..114081 + 222 WP_001641473.1 hypothetical protein -
NBY27_RS23630 (NBY27_23630) 114685..114978 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NBY27_RS23635 (NBY27_23635) 114983..116308 + 1326 WP_086722013.1 type II toxin-antitoxin system HipA family toxin Toxin
NBY27_RS23640 (NBY27_23640) 116369..116575 + 207 WP_000134171.1 hypothetical protein -
NBY27_RS23645 (NBY27_23645) 116677..117087 + 411 WP_000985911.1 hypothetical protein -
NBY27_RS23650 (NBY27_23650) 117100..117915 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NBY27_RS23655 (NBY27_23655) 118169..118594 + 426 WP_001043843.1 hypothetical protein -
NBY27_RS23660 (NBY27_23660) 119048..119284 + 237 Protein_112 type I restriction-modification system subunit M N-terminal domain-containing protein -
NBY27_RS23665 (NBY27_23665) 119339..119638 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / dfrA12 / aadA2 / qacE / sul1 / mph(A) - 1..230317 230317


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50263.22 Da        Isoelectric Point: 6.1932

>T246877 WP_086722013.1 NZ_CP098184:114983-116308 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEERWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT246877 WP_024136327.1 NZ_CP098184:114685-114978 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References