Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3807523..3808177 Replicon chromosome
Accession NZ_CP098183
Organism Escherichia coli strain Z0117EC0133

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag NBY27_RS18515 Protein ID WP_000244772.1
Coordinates 3807523..3807930 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NBY27_RS18520 Protein ID WP_000354046.1
Coordinates 3807911..3808177 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY27_RS18495 (3803480) 3803480..3805213 - 1734 WP_064484803.1 single-stranded-DNA-specific exonuclease RecJ -
NBY27_RS18500 (3805219) 3805219..3805929 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NBY27_RS18505 (3805954) 3805954..3806850 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NBY27_RS18510 (3806962) 3806962..3807483 + 522 WP_001055874.1 flavodoxin FldB -
NBY27_RS18515 (3807523) 3807523..3807930 - 408 WP_000244772.1 protein YgfX Toxin
NBY27_RS18520 (3807911) 3807911..3808177 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NBY27_RS18525 (3808420) 3808420..3809400 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NBY27_RS18530 (3809477) 3809477..3810136 - 660 WP_000250274.1 hemolysin III family protein -
NBY27_RS18535 (3810300) 3810300..3810611 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
NBY27_RS18540 (3810656) 3810656..3812089 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
NBY27_RS18545 (3812146) 3812146..3812889 - 744 WP_021564907.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T246874 WP_000244772.1 NZ_CP098183:c3807930-3807523 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT246874 WP_000354046.1 NZ_CP098183:c3808177-3807911 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References