Detailed information of TA system TA024687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1118489..1119116
• Replicon: chromosome
• Accession: NC_013971
• Organism: Erwinia amylovora ATCC 49946

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: EAM_RS04950
• Protein ID: WP_004156337.1
• Coordinates: 1118489..1118707 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EAM_RS04955
• Protein ID: WP_013035913.1
• Coordinates: 1118727..1119116 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAM_RS04925 (EAM_1004)	1114475..1114813	+	339	WP_004156319.1	P-II family nitrogen regulator	-
EAM_RS04930 (EAM_1005)	1114831..1116132	+	1302	WP_004172214.1	ammonium transporter AmtB	-
EAM_RS04935 (EAM_1006)	1116207..1117067	-	861	WP_004156324.1	acyl-CoA thioesterase II	-
EAM_RS04940 (EAM_1007)	1117292..1117849	+	558	WP_004156333.1	YbaY family lipoprotein	-
EAM_RS04945 (EAM_1008)	1117872..1118189	-	318	WP_004156334.1	MGMT family protein	-
EAM_RS04950 (EAM_1009)	1118489..1118707	-	219	WP_004156337.1	HHA domain-containing protein	Toxin
EAM_RS04955 (EAM_1010)	1118727..1119116	-	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
EAM_RS04960 (EAM_1011)	1119263..1119616	-	354	WP_004156342.1	hypothetical protein	-
EAM_RS04965 (EAM_1014)	1120388..1120534	-	147	WP_004156349.1	type B 50S ribosomal protein L36	-
EAM_RS04970 (EAM_1015)	1120522..1120803	-	282	WP_004156350.1	type B 50S ribosomal protein L31	-
EAM_RS04975 (EAM_1016)	1120951..1124097	-	3147	WP_004161886.1	multidrug efflux RND transporter permease subunit AcrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T24687 WP_004156337.1 NC_013971:c1118707-1118489 [Erwinia amylovora ATCC 49946]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T24687 NC_013971:c1118707-1118489 [Erwinia amylovora ATCC 49946]
ATGACTGACAAACTTCTAACGAAAACTGACTACCTGATGCGCCTCAGGCGTTGTCGTTCAATCGATACACTTGAACGCGT
GATTGAAAAGAACAAGTACGAATTATCTGATGATGAACTGGCTGTATTTTATTCAGCCGCCGATCACCGTCTTGCTGAAC
TAACCATGAATAAGCTGTACGACAAAGTACCGGTCGCTGTCTGGAAGTTTGTCCGTTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT24687 WP_013035913.1 NC_013971:c1119116-1118727 [Erwinia amylovora ATCC 49946]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT24687 NC_013971:c1119116-1118727 [Erwinia amylovora ATCC 49946]
ATGGACGAATACTCACCGAAAAGGCATGATATTGCGCAGCTGAAATTCTTATGTGAAAACCTGTATGATGAAAGTCTGGC
TACGTTAGGCGACAGCCATCATGGCTGGGTTAACGACCCCACCTCAACGAGCAATCTTCAGCTTAACGACCTGATAGAGC
ACATTGCAGCATTCACAATGAATTACAAAATCAAGCATATCGAAGACAGCGATCTGATAAGTCAAATTGATGAGTATCTG
GACGACACCTTTATGCTTTTCAGCAATTACGGTGTCAACAATCTTGACTTACAACGCTGGCAAAAATCAGCGAAGCGATT
GTTTAACATTTTTGCTAAAGAATGCGTAATGAGCCAGATCCAGTCCAGCCATTCATTTAGCAGCCCATAA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0