Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1124157..1124994 Replicon chromosome
Accession NZ_CP098183
Organism Escherichia coli strain Z0117EC0133

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NBY27_RS05230 Protein ID WP_000227784.1
Coordinates 1124157..1124699 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NBY27_RS05235 Protein ID WP_001297137.1
Coordinates 1124683..1124994 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY27_RS05210 (1119696) 1119696..1120607 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
NBY27_RS05215 (1120775) 1120775..1121266 + 492 WP_001138904.1 nucleotide binding protein YajQ -
NBY27_RS05220 (1121394) 1121394..1122758 - 1365 WP_001000974.1 MFS transporter -
NBY27_RS05225 (1123166) 1123166..1124101 + 936 Protein_1005 tetratricopeptide repeat protein -
NBY27_RS05230 (1124157) 1124157..1124699 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NBY27_RS05235 (1124683) 1124683..1124994 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NBY27_RS05240 (1125179) 1125179..1126069 - 891 WP_000971336.1 heme o synthase -
NBY27_RS05245 (1126081) 1126081..1126410 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NBY27_RS05250 (1126410) 1126410..1127024 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NBY27_RS05255 (1127014) 1127014..1129005 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NBY27_RS05260 (1129027) 1129027..1129974 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T246859 WP_000227784.1 NZ_CP098183:c1124699-1124157 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT246859 WP_001297137.1 NZ_CP098183:c1124994-1124683 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References