Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 428390..428887 Replicon chromosome
Accession NZ_CP098183
Organism Escherichia coli strain Z0117EC0133

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NBY27_RS01960 Protein ID WP_001173343.1
Coordinates 428714..428887 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NBY27_RS01955 Protein ID WP_000398619.1
Coordinates 428390..428686 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY27_RS01930 (423860) 423860..424579 - 720 WP_000611288.1 two-component system response regulator DcuR -
NBY27_RS01935 (424576) 424576..426207 - 1632 WP_001216487.1 sensor histidine kinase -
NBY27_RS01945 (427676) 427676..427879 + 204 Protein_364 4Fe-4S mono-cluster protein YjdI -
NBY27_RS01950 (427891) 427891..428163 + 273 WP_000405649.1 GNAT family N-acetyltransferase -
NBY27_RS01955 (428390) 428390..428686 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NBY27_RS01960 (428714) 428714..428887 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NBY27_RS01965 (429006) 429006..430523 - 1518 WP_064484767.1 lysine--tRNA ligase -
NBY27_RS01970 (430760) 430760..432217 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T246856 WP_001173343.1 NZ_CP098183:428714-428887 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT246856 WP_000398619.1 NZ_CP098183:428390-428686 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References