246824

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1218954..1219791 Replicon chromosome
Accession NZ_CP098178
Organism Escherichia coli strain Z0117EC0148

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NBY28_RS05880 Protein ID WP_000227784.1
Coordinates 1218954..1219496 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NBY28_RS05885 Protein ID WP_001297137.1
Coordinates 1219480..1219791 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY28_RS05860 (1214493) 1214493..1215404 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
NBY28_RS05865 (1215572) 1215572..1216063 + 492 WP_001138904.1 nucleotide binding protein YajQ -
NBY28_RS05870 (1216191) 1216191..1217555 - 1365 WP_001000960.1 MFS transporter -
NBY28_RS05875 (1217963) 1217963..1218898 + 936 WP_001368479.1 tetratricopeptide repeat protein -
NBY28_RS05880 (1218954) 1218954..1219496 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NBY28_RS05885 (1219480) 1219480..1219791 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NBY28_RS05890 (1219976) 1219976..1220866 - 891 WP_000971336.1 heme o synthase -
NBY28_RS05895 (1220878) 1220878..1221207 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NBY28_RS05900 (1221207) 1221207..1221821 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NBY28_RS05905 (1221811) 1221811..1223802 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NBY28_RS05910 (1223824) 1223824..1224771 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T246824 WP_000227784.1 NZ_CP098178:c1219496-1218954 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT246824 WP_001297137.1 NZ_CP098178:c1219791-1219480 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References