Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 462973..463470 Replicon chromosome
Accession NZ_CP098178
Organism Escherichia coli strain Z0117EC0148

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NBY28_RS02190 Protein ID WP_001173343.1
Coordinates 463297..463470 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NBY28_RS02185 Protein ID WP_000398619.1
Coordinates 462973..463269 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY28_RS02165 (459704) 459704..460423 - 720 WP_000611288.1 two-component system response regulator DcuR -
NBY28_RS02170 (460420) 460420..462051 - 1632 WP_001216487.1 sensor histidine kinase -
NBY28_RS02175 (462232) 462232..462462 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
NBY28_RS02180 (462474) 462474..462746 + 273 WP_000405649.1 GNAT family N-acetyltransferase -
NBY28_RS02185 (462973) 462973..463269 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NBY28_RS02190 (463297) 463297..463470 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NBY28_RS02195 (463589) 463589..465106 - 1518 WP_001295087.1 lysine--tRNA ligase -
NBY28_RS02200 (465343) 465343..466800 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T246822 WP_001173343.1 NZ_CP098178:463297-463470 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT246822 WP_000398619.1 NZ_CP098178:462973-463269 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References