Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3447364..3448100 Replicon chromosome
Accession NZ_CP098169
Organism Klebsiella pneumoniae strain Z0117KP0003

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag NBY30_RS16695 Protein ID WP_032433360.1
Coordinates 3447618..3448100 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag NBY30_RS16690 Protein ID WP_003026799.1
Coordinates 3447364..3447630 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY30_RS16665 (3443010) 3443010..3444149 + 1140 WP_032433368.1 mannitol dehydrogenase -
NBY30_RS16670 (3444178) 3444178..3444840 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
NBY30_RS16675 (3444821) 3444821..3445828 + 1008 WP_280954017.1 dihydroxyacetone kinase subunit DhaK -
NBY30_RS16680 (3445846) 3445846..3446478 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
NBY30_RS16685 (3446488) 3446488..3447051 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
NBY30_RS16690 (3447364) 3447364..3447630 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
NBY30_RS16695 (3447618) 3447618..3448100 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
NBY30_RS16700 (3448300) 3448300..3449703 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
NBY30_RS16705 (3449732) 3449732..3450364 - 633 WP_250833685.1 hypothetical protein -
NBY30_RS16710 (3450744) 3450744..3451343 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
NBY30_RS16715 (3451556) 3451556..3452500 - 945 WP_077254249.1 fimbrial protein -
NBY30_RS16720 (3452512) 3452512..3453090 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3416249..3461326 45077
- flank IS/Tn - - 3448300..3449703 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T246799 WP_032433360.1 NZ_CP098169:3447618-3448100 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT246799 WP_003026799.1 NZ_CP098169:3447364-3447630 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References