Detailed information of TA system TA024676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1081001..1081628
• Replicon: chromosome
• Accession: NC_013961
• Organism: Erwinia amylovora CFBP1430

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: EAMY_RS22135
• Protein ID: WP_004156337.1
• Coordinates: 1081001..1081219 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EAMY_RS22140
• Protein ID: WP_013035913.1
• Coordinates: 1081239..1081628 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAMY_RS22110	1076987..1077325	+	339	WP_004156319.1	P-II family nitrogen regulator	-
EAMY_RS22115	1077343..1078644	+	1302	WP_004172214.1	ammonium transporter AmtB	-
EAMY_RS22120	1078719..1079579	-	861	WP_004156324.1	acyl-CoA thioesterase II	-
EAMY_RS22125	1079804..1080361	+	558	WP_004156333.1	YbaY family lipoprotein	-
EAMY_RS22130	1080384..1080701	-	318	WP_004156334.1	MGMT family protein	-
EAMY_RS22135	1081001..1081219	-	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
EAMY_RS22140	1081239..1081628	-	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
EAMY_RS22145	1081775..1082128	-	354	WP_004156342.1	hypothetical protein	-
EAMY_RS22150	1082900..1083046	-	147	WP_004156349.1	type B 50S ribosomal protein L36	-
EAMY_RS22155	1083034..1083315	-	282	WP_004156350.1	type B 50S ribosomal protein L31	-
EAMY_RS22160	1083463..1086609	-	3147	WP_004156353.1	multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T24676 WP_004156337.1 NC_013961:c1081219-1081001 [Erwinia amylovora CFBP1430]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T24676 NC_013961:c1081219-1081001 [Erwinia amylovora CFBP1430]
ATGACTGACAAACTTCTAACGAAAACTGACTACCTGATGCGCCTCAGGCGTTGTCGTTCAATCGATACACTTGAACGCGT
GATTGAAAAGAACAAGTACGAATTATCTGATGATGAACTGGCTGTATTTTATTCAGCCGCCGATCACCGTCTTGCTGAAC
TAACCATGAATAAGCTGTACGACAAAGTACCGGTCGCTGTCTGGAAGTTTGTCCGTTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT24676 WP_013035913.1 NC_013961:c1081628-1081239 [Erwinia amylovora CFBP1430]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT24676 NC_013961:c1081628-1081239 [Erwinia amylovora CFBP1430]
ATGGACGAATACTCACCGAAAAGGCATGATATTGCGCAGCTGAAATTCTTATGTGAAAACCTGTATGATGAAAGTCTGGC
TACGTTAGGCGACAGCCATCATGGCTGGGTTAACGACCCCACCTCAACGAGCAATCTTCAGCTTAACGACCTGATAGAGC
ACATTGCAGCATTCACAATGAATTACAAAATCAAGCATATCGAAGACAGCGATCTGATAAGTCAAATTGATGAGTATCTG
GACGACACCTTTATGCTTTTCAGCAATTACGGTGTCAACAATCTTGACTTACAACGCTGGCAAAAATCAGCGAAGCGATT
GTTTAACATTTTTGCTAAAGAATGCGTAATGAGCCAGATCCAGTCCAGCCATTCATTTAGCAGCCCATAA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0