Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5310695..5311320 Replicon chromosome
Accession NZ_CP098159
Organism Klebsiella pneumoniae strain Z0117KP0008

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NBY40_RS25625 Protein ID WP_002882817.1
Coordinates 5310695..5311078 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NBY40_RS25630 Protein ID WP_004150355.1
Coordinates 5311078..5311320 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY40_RS25610 (5308061) 5308061..5308963 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NBY40_RS25615 (5308960) 5308960..5309595 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NBY40_RS25620 (5309592) 5309592..5310521 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NBY40_RS25625 (5310695) 5310695..5311078 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NBY40_RS25630 (5311078) 5311078..5311320 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NBY40_RS25635 (5311525) 5311525..5312442 + 918 WP_009484979.1 alpha/beta hydrolase -
NBY40_RS25640 (5312456) 5312456..5313397 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NBY40_RS25645 (5313442) 5313442..5313879 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NBY40_RS25650 (5313876) 5313876..5314736 - 861 WP_004146232.1 virulence factor BrkB family protein -
NBY40_RS25655 (5314730) 5314730..5315329 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T246739 WP_002882817.1 NZ_CP098159:c5311078-5310695 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT246739 WP_004150355.1 NZ_CP098159:c5311320-5311078 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References