Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5282897..5283522 Replicon chromosome
Accession NZ_CP098149
Organism Klebsiella pneumoniae strain Z0117KP0019

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NBY46_RS25605 Protein ID WP_002882817.1
Coordinates 5282897..5283280 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NBY46_RS25610 Protein ID WP_004150355.1
Coordinates 5283280..5283522 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY46_RS25590 (5280263) 5280263..5281165 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NBY46_RS25595 (5281162) 5281162..5281797 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NBY46_RS25600 (5281794) 5281794..5282723 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NBY46_RS25605 (5282897) 5282897..5283280 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NBY46_RS25610 (5283280) 5283280..5283522 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NBY46_RS25615 (5283727) 5283727..5284644 + 918 WP_009484979.1 alpha/beta hydrolase -
NBY46_RS25620 (5284658) 5284658..5285599 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NBY46_RS25625 (5285644) 5285644..5286081 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NBY46_RS25630 (5286078) 5286078..5286938 - 861 WP_004146232.1 virulence factor BrkB family protein -
NBY46_RS25635 (5286932) 5286932..5287531 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T246724 WP_002882817.1 NZ_CP098149:c5283280-5282897 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT246724 WP_004150355.1 NZ_CP098149:c5283522-5283280 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References