Detailed information of TA system TA024671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3506459..3508173
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: D5C184
• Locus tag: NHAL_RS16535
• Protein ID: WP_013034285.1
• Coordinates: 3506459..3507112 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: D5C185
• Locus tag: NHAL_RS16540
• Protein ID: WP_013034286.1
• Coordinates: 3507112..3508173 (+)
• Length: 354 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS16515	3501600..3504324	+	2725	Protein_3287	N-6 DNA methylase	-
NHAL_RS19935	3504317..3505612	+	1296	WP_013034283.1	restriction endonuclease subunit S	-
NHAL_RS16530	3505761..3506381	+	621	WP_157862673.1	nucleotidyltransferase domain-containing protein	-
NHAL_RS16535	3506459..3507112	+	654	WP_013034285.1	DUF4433 domain-containing protein	Toxin
NHAL_RS16540	3507112..3508173	+	1062	WP_013034286.1	macro domain-containing protein	Antitoxin
NHAL_RS16545	3508188..3511537	+	3350	Protein_3292	type I restriction endonuclease subunit R	-
NHAL_RS16550	3511534..3512220	+	687	WP_013034287.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3503219..3511537	8318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25195.07 Da
• Isoelectric Point: 9.5756

>T24671 WP_013034285.1 NC_013960:3506459-3507112 [Nitrosococcus halophilus Nc 4]
MSTPITYNLNPVKGLIFRITHRDNILWILDHGLHCPNGTVFDHNFVPIGNPELIDRRHHREVPIPPGGVLGDYVPFYFTP
YSPMMYNIKTGYGGVKKRRNDEIVVLVSSLHQVAKEQRKFVFTDRHAYLRAANFYNNINDLNLVDFKLLRSRDFSRDVED
PEKIERYQAEALVHQSLPVQALLGIVCYTKAVADEVQSVLRVRKITMRTLVKPSWYF

Nucleotide

• Length: 654 bp

>T24671 NC_013960:3506459-3507112 [Nitrosococcus halophilus Nc 4]
ATGAGTACCCCAATCACCTACAATCTCAATCCAGTGAAGGGGCTGATCTTCCGCATCACGCATCGCGACAATATCCTCTG
GATTCTAGATCATGGTCTGCACTGTCCCAACGGGACAGTCTTCGACCATAACTTTGTACCCATCGGCAATCCAGAACTGA
TTGACAGACGCCATCACCGAGAGGTCCCAATTCCGCCGGGCGGTGTGCTTGGTGATTATGTTCCATTTTACTTCACGCCC
TATTCACCCATGATGTACAACATCAAAACGGGCTATGGCGGTGTCAAAAAGAGGCGCAATGATGAAATCGTGGTATTAGT
CAGCTCATTGCATCAAGTTGCTAAAGAGCAGAGGAAATTTGTGTTTACTGATCGCCACGCGTACCTACGAGCAGCAAATT
TTTATAATAATATTAATGACTTGAATCTTGTTGACTTTAAACTGCTGCGTAGCCGGGATTTTAGCCGTGACGTTGAGGAT
CCGGAAAAAATCGAGCGCTACCAGGCCGAAGCACTGGTCCACCAATCCCTCCCAGTACAGGCCTTATTGGGCATCGTTTG
CTACACTAAGGCTGTAGCCGATGAGGTGCAGTCTGTTCTTCGAGTTCGCAAGATAACGATGAGAACGCTCGTGAAACCAA
GCTGGTACTTCTGA

Antitoxin

Protein

• Length: 354 a.a.
• Molecular weight: 40040.48 Da
• Isoelectric Point: 8.4328

>AT24671 WP_013034286.1 NC_013960:3507112-3508173 [Nitrosococcus halophilus Nc 4]
MIEYTKGNLLKADVEALVNTVNTVGIMGKGIALMFKEAFKDNFDAYAAACKAGEVKIGQMFTTETGNLMGPQWIINFPTK
KHWRNKTKLAWIEEGLEDLRRTIIKYGIDSIAIPPLGCGNGGLDWREVRPLIEKCLVDLNGIHILVYEPTQKYQNVAKRK
GVQTLTPARALIAELVRRYWVLGIECSLLEIQKLAWFLERRLEESGLDNPLQLDFKANRYGPYADRLRHLLDALDGSYLR
CDKRLADAKPEDTIAFNDTQKEYVATYLKSKAKAYLPALEATTKLIDGFESPLGMELLATVDWLLVREHCKPEVEDVKRG
IAHWPASRSAALRKQRIFDDRLISLALERLAAY

Nucleotide

• Length: 1062 bp

>AT24671 NC_013960:3507112-3508173 [Nitrosococcus halophilus Nc 4]
ATGATCGAGTATACCAAAGGTAATCTGCTGAAAGCCGATGTGGAAGCCCTGGTCAACACCGTCAATACGGTCGGCATAAT
GGGTAAAGGTATTGCTTTGATGTTCAAAGAAGCCTTCAAGGATAACTTTGATGCCTATGCCGCGGCTTGCAAAGCCGGTG
AAGTCAAAATCGGGCAGATGTTCACGACTGAAACCGGCAACCTGATGGGACCGCAGTGGATCATCAATTTCCCCACCAAA
AAACACTGGCGCAATAAAACAAAGCTGGCATGGATTGAGGAAGGCCTAGAGGATCTGCGTCGCACCATTATCAAATACGG
GATTGACTCTATTGCCATCCCCCCCCTGGGTTGCGGCAATGGCGGCTTGGATTGGCGCGAGGTAAGACCACTCATTGAAA
AGTGCTTGGTAGATCTGAATGGTATTCATATTCTGGTCTATGAACCCACTCAGAAATATCAGAATGTTGCCAAACGCAAG
GGTGTACAAACATTGACCCCTGCGCGTGCCCTGATAGCCGAACTCGTCCGGCGCTATTGGGTGCTGGGCATTGAATGCAG
CCTGCTCGAAATCCAAAAATTAGCATGGTTCCTGGAACGTCGTCTGGAAGAAAGCGGATTAGATAATCCACTCCAGCTTG
ATTTTAAAGCCAATCGTTATGGCCCCTATGCTGACCGGCTGCGCCACTTGTTGGATGCCCTGGATGGTAGTTATCTGCGC
TGCGACAAGCGATTGGCCGATGCCAAACCCGAGGATACGATCGCTTTCAATGATACCCAAAAAGAATATGTGGCAACCTA
CCTGAAAAGTAAAGCCAAGGCCTATCTGCCGGCGCTGGAGGCAACGACAAAACTGATTGATGGCTTCGAGTCGCCACTCG
GTATGGAGCTACTGGCTACCGTCGATTGGTTGTTAGTCCGCGAGCACTGCAAACCCGAAGTAGAGGATGTTAAACGAGGC
ATTGCCCATTGGCCCGCTAGCCGAAGTGCCGCACTTAGGAAACAACGCATATTTGATGATCGTTTAATCAGCTTGGCACT
CGAACGCCTAGCCGCCTACTGA

Structures

Toxin

• AlphaFold DB: D5C184

Antitoxin

• AlphaFold DB: D5C185