Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 44672..45297 Replicon chromosome
Accession NZ_CP098145
Organism Klebsiella pneumoniae strain Z0117KP0020

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NBY48_RS00205 Protein ID WP_002882817.1
Coordinates 44914..45297 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NBY48_RS00200 Protein ID WP_004150355.1
Coordinates 44672..44914 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY48_RS00175 (40663) 40663..41262 + 600 WP_004151865.1 glucose-1-phosphatase -
NBY48_RS00180 (41256) 41256..42116 + 861 WP_004146232.1 virulence factor BrkB family protein -
NBY48_RS00185 (42113) 42113..42550 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NBY48_RS00190 (42595) 42595..43536 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NBY48_RS00195 (43550) 43550..44467 - 918 WP_009484979.1 alpha/beta hydrolase -
NBY48_RS00200 (44672) 44672..44914 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NBY48_RS00205 (44914) 44914..45297 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NBY48_RS00210 (45471) 45471..46400 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NBY48_RS00215 (46397) 46397..47032 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NBY48_RS00220 (47029) 47029..47931 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T246692 WP_002882817.1 NZ_CP098145:44914-45297 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT246692 WP_004150355.1 NZ_CP098145:44672-44914 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References